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2.A.104.1.1
The L-alanine exporter, AlaE (YgaW). (In the DUF1144 or PF06610 superfamily) (149aas; 4 TMSs) (Hori et al., 2011).  Two charged residues are essential for it's efflux activity (Kim et al. 2016).

Accession Number:A8ANM6
Protein Name:L-alanine exporter AlaE
Length:149
Molecular Weight:16685.00
Species:Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) [290338]
Number of TMSs:4
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Alanine

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MFSPQSRLRH AVADTFAMVV YCSVVNMLIE IFLSGMTFEQ SLSSRLVAIP VNILIAWPYG 
61:	MYRDAIMRVA RKASPASWVK NLADVLAYVT FQSPVYVAIL LTVGADWHQI TAAVSSNIVI 
121:	SMLMGAVYGY FLDYCRRLFK VSSYHQAKA