TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


2.A.11.1.1
[Citrate or D-isocitrate]•M2+ (Mg2+ preferring):H+ symporter, CitM (transports Mg2+, Mn2+, Ni2+, Co2+ & Zn2+).  Regulated by the CitS/CitT two component system (Yamamoto et al. 2000; Repizo et al. 2006).

Accession Number:P55069
Protein Name:CitM
Length:433
Molecular Weight:45830.00
Species:Bacillus subtilis [1423]
Number of TMSs:13
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Mg2+, Mn2+, Ni2+, Co2+, Zn2+, H+

Cross database links:

HEGENOM: HBG297065 HBG297065
RefSeq: NP_388642.1   
Entrez Gene ID: 938801   
Pfam: PF03600   
KEGG: bsu:BSU07610    bsu:BSU07610   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015137 F:citrate transmembrane transporter activity
GO:0015293 F:symporter activity
GO:0006101 P:citrate metabolic process
GO:0015746 P:citrate transport
GO:0055085 P:transmembrane transport

References (14)

[1] “Secondary transporters for citrate and the Mg(2+)-citrate complex in Bacillus subtilis are homologous proteins.”  Boorsma A.et.al.   8892821
[2] “Cloning and sequencing of a 35.7 kb in the 70 degree-73 degree region of the Bacillus subtilis genome reveal genes for a new two-component system, three spore germination proteins, an iron uptake system and a general stress response protein.”  Yamamoto H.et.al.   9272861
[3] “The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.”  Kunst F.et.al.   9384377
[4] “Catabolite repression and induction of the Mg(2+)-citrate transporter CitM of Bacillus subtilis.”  Warner J.B.et.al.   11029430
[5] “The CitST two-component system regulates the expression of the Mg-citrate transporter in Bacillus subtilis.”  Yamamoto H.et.al.   10972810
[6] “Complementary metal ion specificity of the metal-citrate transporters CitM and CitH of Bacillus subtilis.”  Krom B.P.et.al.   11053381
[7] “Functional characterization of CitM, the Mg2+-citrate transporter.”  Li H.et.al.   11891560
[8] “Secondary transporters for citrate and the Mg(2+)-citrate complex in Bacillus subtilis are homologous proteins.”  Boorsma A.et.al.   8892821
[9] “Cloning and sequencing of a 35.7 kb in the 70 degree-73 degree region of the Bacillus subtilis genome reveal genes for a new two-component system, three spore germination proteins, an iron uptake system and a general stress response protein.”  Yamamoto H.et.al.   9272861
[10] “The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.”  Kunst F.et.al.   9384377
[11] “Catabolite repression and induction of the Mg(2+)-citrate transporter CitM of Bacillus subtilis.”  Warner J.B.et.al.   11029430
[12] “The CitST two-component system regulates the expression of the Mg-citrate transporter in Bacillus subtilis.”  Yamamoto H.et.al.   10972810
[13] “Complementary metal ion specificity of the metal-citrate transporters CitM and CitH of Bacillus subtilis.”  Krom B.P.et.al.   11053381
[14] “Functional characterization of CitM, the Mg2+-citrate transporter.”  Li H.et.al.   11891560

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MLAILGFLMM LVFMALIMTK RLSVLTALVL TPIVFALIAG FGFTEVGDMM ISGIQQVAPT 
61:	AVMIMFAILY FGIMIDTGLF DPMVGKILSM VKGDPLKIVV GTAVLTMLVA LDGDGSTTYM 
121:	ITTSAMLPLY LLLGIRPIIL AGIAGVGMGI MNTIPWGGAT PRAASALGVD PAELTGPMIP 
181:	VIASGMLCMV AVAYVLGKAE RKRLGVIELK QPANANEPAA AVEDEWKRPK LWWFNLLLTL 
241:	SLIGCLVSGK VSLTVLFVIA FCIALIVNYP NLEHQRQRIA AHSSNVLAIG SMIFAAGVFT 
301:	GILTGTKMVD EMAISLVSMI PEQMGGLIPA IVALTSGIFT FLMPNDAYFY GVLPILSETA 
361:	VAYGVDKVEI ARASIIGQPI HMLSPLVPST HLLVGLVGVS IDDHQKFALK WAVLAVIVMT 
421:	AIALLIGAIS ISV