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2.A.13.1.1
Anaerobic C4-dicarboxylate antiporter (aspartate:fumarate antiporter).  Can catalyze both uptake and exchange (Zientz et al. 1996).

Accession Number:P0ABN5
Protein Name:Anaerobic C4-dicarboxylate transporter DcuA aka GENA aka B4138
Length:433
Molecular Weight:45751.00
Species:Escherichia coli [83333]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate aspartate, Fumarate

Cross database links:

Genevestigator: P0ABN5
EchoBASE: EB1207
EcoGene: EG11225
eggNOG: COG2704
HEGENOM: HBG297083
RefSeq: AP_004639.1    NP_418561.1   
Entrez Gene ID: 948659   
Pfam: PF03605   
BioCyc: EcoCyc:DCUA-MONOMER    ECOL168927:B4138-MONOMER   
KEGG: ecj:JW5735    eco:b4138   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015556 F:C4-dicarboxylate transmembrane transporter ...
GO:0015740 P:C4-dicarboxylate transport

References (10)

[1] “Construction and properties of Escherichia coli mutants defective in two genes encoding homologous membrane proteins with putative roles in anaerobic C4-dicarboxylic acid transport.”  Six S.et.al.   8131924
[2] “Escherichia coli possesses two homologous anaerobic C4-dicarboxylate membrane transporters (DcuA and DcuB) distinct from the aerobic dicarboxylate transport system (Dct).”  Six S.et.al.   7961398
[3] “Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes.”  Burland V.D.et.al.   7610040
[4] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[5] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[6] “Cloning and nucleotide sequence of the aspartase gene of Escherichia coli W.”  Takagi J.S.et.al.   2987841
[7] “Structural and functional relationships between fumarase and aspartase. Nucleotide sequences of the fumarase (fumC) and aspartase (aspA) genes of Escherichia coli K12.”  Woods S.A.et.al.   3541901
[8] “Nucleotide sequence of the FNR-regulated fumarase gene (fumB) of Escherichia coli K-12.”  Bell P.J.et.al.   2656658
[9] “Topological analysis of DcuA, an anaerobic C4-dicarboxylate transporter of Escherichia coli.”  Golby P.et.al.   9733683
[10] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MLVVELIIVL LAIFLGARLG GIGIGFAGGL GVLVLAAIGV KPGNIPFDVI SIIMAVIAAI 
61:	SAMQVAGGLD YLVHQTEKLL RRNPKYITIL APIVTYFLTI FAGTGNISLA TLPVIAEVAK 
121:	EQGVKPCRPL STAVVSAQIA ITASPISAAV VYMSSVMEGH GISYLHLLSV VIPSTLLAVL 
181:	VMSFLVTMLF NSKLSDDPIY RKRLEEGLVE LRGEKQIEIK SGAKTSVWLF LLGVVGVVIY 
241:	AIINSPSMGL VEKPLMNTTN AILIIMLSVA TLTTVICKVD TDNILNSSTF KAGMSACICI 
301:	LGVAWLGDTF VSNNIDWIKD TAGEVIQGHP WLLAVIFFFA SALLYSQAAT AKALMPMALA 
361:	LNVSPLTAVA SFAAVSGLFI LPTYPTLVAA VQMDDTGTTR IGKFVFNHPF FIPGTLGVAL 
421:	AVCFGFVLGS FML