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2.A.13.1.2
Anaerobic C4-dicarboxylate uptake/efflux porter, DcuB. Also catalyzes substrate:substrate antiport (fumarate:malate or fumarate:succinate antiport), but it is also a cosensor for the sensor kinase, DcuS (Kleefeld et al. 2009; Bauer et al. 2011; Chen et al. 2014) It has 12 established TMSs where the loop between TMSs 11 and 12 is the sensor. A central water-filled cavity may provide the transport pathway. The sensory domain of DcuB is composed of cytoplasmic loop XI/XII and a membrane integral region with the regulatory residues Thr396/Asp398 and Lys353. Also transporter D-tartrate (Bauer et al. 2011).  Also transports D-tartrate (Kim et al. 2007).  The DcuB-DcuS interaction has been reviewed (Bauer et al. 2011; Chen et al. 2014) It has 12 established TMSs where the loop between TMSs 11 and 12 is the sensor. A central water-filled cavity may provide the transport pathway. The sensory domain of DcuB is composed of cytoplasmic loop XI/XII and a membrane integral region with the regulatory residues Thr396/Asp398 and Lys353. Also transporter D-tartrate (Bauer et al. 2011).  Also transports D-tartrate (Kim et al. 2007).  The DcuB-DcuS interaction has been reviewed (Unden et al. 2016).

Accession Number:P0ABN9
Protein Name:Anaerobic C4-dicarboxylate transporter DcuB aka GENF aka B4123
Length:446
Molecular Weight:47935.00
Species:Escherichia coli [83333]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Fumarate, Succinate, malate

Cross database links:

Genevestigator: P0ABN9
EchoBASE: EB0006
EcoGene: EG10006
eggNOG: COG2704
HEGENOM: HBG297083
RefSeq: AP_004624.1    NP_418547.1   
Entrez Gene ID: 948641   
Pfam: PF03605   
BioCyc: EcoCyc:DCUB-MONOMER    ECOL168927:B4123-MONOMER   
KEGG: ecj:JW4084    eco:b4123   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015556 F:C4-dicarboxylate transmembrane transporter ...
GO:0015740 P:C4-dicarboxylate transport

References (7)

[1] “Construction and properties of Escherichia coli mutants defective in two genes encoding homologous membrane proteins with putative roles in anaerobic C4-dicarboxylic acid transport.”  Six S.et.al.   8131924
[2] “Escherichia coli possesses two homologous anaerobic C4-dicarboxylate membrane transporters (DcuA and DcuB) distinct from the aerobic dicarboxylate transport system (Dct).”  Six S.et.al.   7961398
[3] “Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes.”  Burland V.D.et.al.   7610040
[4] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[5] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[6] “Nucleotide sequence of the FNR-regulated fumarase gene (fumB) of Escherichia coli K-12.”  Bell P.J.et.al.   2656658
[7] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

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FASTA formatted sequence
1:	MLFTIQLIII LICLFYGARK GGIALGLLGG IGLVILVFVF HLQPGKPPVD VMLVIIAVVA 
61:	ASATLQASGG LDVMLQIAEK LLRRNPKYVS IVAPFVTCTL TILCGTGHVV YTILPIIYDV 
121:	AIKNNIRPER PMAASSIGAQ MGIIASPVSV AVVSLVAMLG NVTFDGRHLE FLDLLAITIP 
181:	STLIGILAIG IFSWFRGKDL DKDEEFQKFI SVPENREYVY GDTATLLDKK LPKSNWLAMW 
241:	IFLGAIAVVA LLGADSDLRP SFGGKPLSMV LVIQMFMLLT GALIIILTKT NPASISKNEV 
301:	FRSGMIAIVA VYGIAWMAET MFGAHMSEIQ GVLGEMVKEY PWAYAIVLLL VSKFVNSQAA 
361:	ALAAIVPVAL AIGVDPAYIV ASAPACYGYY ILPTYPSDLA AIQFDRSGTT HIGRFVINHS 
421:	FILPGLIGVS VSCVFGWIFA AMYGFL