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2.A.14.1.2
Glycolate permease, GlcA or YghK (substrates: L-lactate, D-lactate and glycolate). 
Encoded in an operon with malate synthase and glycolate oxidase (Moraes and Reithmeier 2012).

Accession Number:Q46839
Protein Name:YghK aka GLCA aka B2975
Length:560
Molecular Weight:58920.00
Species:Escherichia coli [83333]
Number of TMSs:18
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Lactate, Glycolate

Cross database links:

Genevestigator: Q46839
EchoBASE: EB2818
EcoGene: EG12995
eggNOG: COG1620
HEGENOM: HBG289840
DIP: DIP-12206N
RefSeq: AP_003529.1    NP_417449.1   
Entrez Gene ID: 947259   
Pfam: PF02652   
BioCyc: EcoCyc:B2975-MONOMER    ECOL168927:B2975-MONOMER   
KEGG: ecj:JW2942    eco:b2975   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015129 F:lactate transmembrane transporter activity
GO:0015727 P:lactate transport

References (5)

[1] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[2] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[3] “The gene yghK linked to the glc operon of Escherichia coli encodes a permease for glycolate that is structurally and functionally similar to L-lactate permease.”  Nunez M.F.et.al.   11283302
[4] “Transport of L-lactate, D-lactate, and glycolate by the LldP and GlcA membrane carriers of Escherichia coli.”  Nunez M.F.et.al.   11785976
[5] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MVTWTQMYMP MGGLGLSALV ALIPIIFFFV ALAVLRLKGH VAGAITLILS ILIAIFAFKM 
61:	PIDMAFAAAG YGFIYGLWPI AWIIVAAVFL YKLTVASGQF DIIRSSVISI TDDQRLQVLL 
121:	IGFSFGALLE GAAGFGAPVA ITGALLVGLG FKPLYAAGLC LIANTAPVAF GALGVPILVA 
181:	GQVTGIDPFH IGAMAGRQLP FLSVLVPFWL VAMMDGWKGV KETWPAALVA GGSFAVTQFF 
241:	TSNYIGPELP DITSALVSIV SLALFLKVWR PKNTETAISM GQSAGAMVVN KPSSGGPVPS 
301:	EYSLGQIIRA WSPFLILTVL VTIWTMKPFK ALFAPGGAFY SLVINFQIPH LHQQVLKAAP 
361:	IVAQPTPMDA VFKFDPLSAG GTAIFIAAII SIFILGVGIK KGIGVFAETL ISLKWPILSI 
421:	GMVLAFAFVT NYSGMSTTLA LVLAGTGVMF PFFSPFLGWL GVFLTGSDTS SNALFGSLQS 
481:	TTAQQINVSD TLLVAANTSG GVTGKMISPQ SIAVACAATG MVGRESELFR YTVKHSLIFA 
541:	SVIGIITLLQ AYVFTGMLVS