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2.A.17.1.2
The di/tripeptide:H+ symport permease, TppB (DtpA or YdgR) (transports di and tripeptides and peptidomimetics such as aminocephalosporins (Weitz et al., 2007).  The transporter has two alternate conformations, one of which is promoted by inhbitor binding (Bippes et al. 2013).

Accession Number:P77304
Protein Name:tppB
Length:500
Molecular Weight:53991.00
Species:Escherichia coli (strain K12) [83333]
Number of TMSs:14
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate dipeptides, tripeptides, H+, Aminocephalosporins

Cross database links:

Genevestigator: P77304
EchoBASE: EB3698
EcoGene: EG13939
eggNOG: COG3104
HEGENOM: HBG586603
RefSeq: AP_002256.1    NP_416151.1   
Entrez Gene ID: 947436   
Pfam: PF00854   
BioCyc: EcoCyc:B1634-MONOMER    ECOL168927:B1634-MONOMER   
KEGG: ecj:JW1626    eco:b1634   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0042936 F:dipeptide transporter activity
GO:0015333 F:peptide:hydrogen symporter activity
GO:0042937 F:tripeptide transporter activity
GO:0042938 P:dipeptide transport
GO:0015031 P:protein transport
GO:0042939 P:tripeptide transport

References (6)

[1] “A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map.”  Aiba H.et.al.   9097039
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “The Escherichia coli tppB (ydgR) gene represents a new class of OmpR-regulated genes.”  Goh E.B.et.al.   15175316
[5] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996
[6] “Functional and structural characterization of a prokaryotic peptide transporter with features similar to mammalian PEPT1.”  Weitz D.et.al.   17158458

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSTANQKPTE SVSLNAFKQP KAFYLIFSIE LWERFGYYGL QGIMAVYLVK QLGMSEADSI 
61:	TLFSSFSALV YGLVAIGGWL GDKVLGTKRV IMLGAIVLAI GYALVAWSGH DAGIVYMGMA 
121:	AIAVGNGLFK ANPSSLLSTC YEKNDPRLDG AFTMYYMSVN IGSFFSMIAT PWLAAKYGWS 
181:	VAFALSVVGL LITIVNFAFC QRWVKQYGSK PDFEPINYRN LLLTIIGVVA LIAIATWLLH 
241:	NQEVARMALG VVAFGIVVIF GKEAFAMKGA ARRKMIVAFI LMLEAIIFFV LYSQMPTSLN 
301:	FFAIRNVEHS ILGLAVEPEQ YQALNPFWII IGSPILAAIY NKMGDTLPMP TKFAIGMVMC 
361:	SGAFLILPLG AKFASDAGIV SVSWLVASYG LQSIGELMIS GLGLAMVAQL VPQRLMGFIM 
421:	GSWFLTTAGA NLIGGYVAGM MAVPDNVTDP LMSLEVYGRV FLQIGVATAV IAVLMLLTAP 
481:	KLHRMTQDDA ADKAAKAAVA