TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


2.A.17.3.1
Dual affinity Nitrate/Chlorate symporter, Nrt1.1; CHL1 (Martin et al., 2008).  The low affinity form is a homo-dimer and has Thr101 in the non-phosphorylated form; the high affinty form (0.1 micromolar Km) is a monomer and has Thr101 phosphorylated (Sun and Zheng 2015).

Accession Number:Q05085
Protein Name:CHL1 aka AT1G12110 aka T28K15_13 aka F12F1.1
Length:590
Molecular Weight:64922.00
Species:Arabidopsis thaliana (Mouse-ear cress) [3702]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate nitrate, Chlorate

Cross database links:

Genevestigator: Q05085
eggNOG: KOG1237
HEGENOM: HBG605267
RefSeq: NP_563899.1   
Entrez Gene ID: 837763   
Pfam: PF00854   
KEGG: ath:AT1G12110   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015112 F:nitrate transmembrane transporter activity
GO:0005515 F:protein binding
GO:0015293 F:symporter activity
GO:0042128 P:nitrate assimilation
GO:0015706 P:nitrate transport
GO:0006857 P:oligopeptide transport
GO:0009635 P:response to herbicide
GO:0010167 P:response to nitrate
GO:0009414 P:response to water deprivation

References (3)

[1] “The herbicide sensitivity gene CHL1 of Arabidopsis encodes a nitrate-inducible nitrate transporter.”  Tsay Y.-F.et.al.   8453665
[2] “Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.”  Theologis A.et.al.   11130712
[3] “Empirical analysis of transcriptional activity in the Arabidopsis genome.”  Yamada K.et.al.   14593172
Structure:
4OH3   5A2N   5A2O     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MSLPETKSDD ILLDAWDFQG RPADRSKTGG WASAAMILCI EAVERLTTLG IGVNLVTYLT 
61:	GTMHLGNATA ANTVTNFLGT SFMLCLLGGF IADTFLGRYL TIAIFAAIQA TGVSILTLST 
121:	IIPGLRPPRC NPTTSSHCEQ ASGIQLTVLY LALYLTALGT GGVKASVSGF GSDQFDETEP 
181:	KERSKMTYFF NRFFFCINVG SLLAVTVLVY VQDDVGRKWG YGICAFAIVL ALSVFLAGTN 
241:	RYRFKKLIGS PMTQVAAVIV AAWRNRKLEL PADPSYLYDV DDIIAAEGSM KGKQKLPHTE 
301:	QFRSLDKAAI RDQEAGVTSN VFNKWTLSTL TDVEEVKQIV RMLPIWATCI LFWTVHAQLT 
361:	TLSVAQSETL DRSIGSFEIP PASMAVFYVG GLLLTTAVYD RVAIRLCKKL FNYPHGLRPL 
421:	QRIGLGLFFG SMAMAVAALV ELKRLRTAHA HGPTVKTLPL GFYLLIPQYL IVGIGEALIY 
481:	TGQLDFFLRE CPKGMKGMST GLLLSTLALG FFFSSVLVTI VEKFTGKAHP WIADDLNKGR 
541:	LYNFYWLVAV LVALNFLIFL VFSKWYVYKE KRLAEVGIEL DDEPSIPMGH