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2.A.17.3.17
Transporter for glucosinolates (aliphatic but not indole glucosinolates such as 4-methylthiobutyl glucosinolate, major defence compounds, translocated to seeds on maturation) as well as gibberellic acid and jasmonoyl-L-isoleucine, GTR1 or NPF2.10, of 636 aas and 12 TMSs (Nour-Eldin et al. 2012; Ishimaru et al. 2017). Regulated at the transcriptional level, but also postranslationally.  Dimerization of GTR1, possibly induced by dephosphorylation of a Thr residue, regulates its plasma membrane localization, leading to increased transport of glucosinolates and gibberellic acid (Ishimaru et al. 2017).

Accession Number:Q944G5
Protein Name:Probable peptide/nitrate transporter At3g47960
Length:636
Molecular Weight:71040.00
Species:Arabidopsis thaliana (Mouse-ear cress) [3702]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate 4-methylthiobutyl glucosinolate, jasmonoyl-L-isoleucine, gibberellic acid, Alkyl glucosinolates

Cross database links:

Genevestigator: Q944G5
eggNOG: NOG259627
HEGENOM: HOG000237399
Entrez Gene ID: 823951   
Pfam: PF00854   
KEGG: ath:AT3G47960   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005215 F:transporter activity
GO:0006857 P:oligopeptide transport

References (6)

[1] “Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.”  Salanoubat M.et.al.   11130713
[2] “Empirical analysis of transcriptional activity in the Arabidopsis genome.”  Yamada K.et.al.   14593172
[3] “Large-scale analysis of in vivo phosphorylated membrane proteins by immobilized metal ion affinity chromatography and mass spectrometry.”  Nuehse T.S.et.al.   14506206
[4] “Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database.”  Nuehse T.S.et.al.   15308754
[5] “Nitrate transporters and peptide transporters.”  Tsay Y.F.et.al.   17481610
[6] “The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal from the xylem sap and mediates cadmium tolerance.”  Li J.Y.et.al.   20501909

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MKSRVILNHR DRRDKNHNNN NTNHYTQVDT MERKPLEVEP STTTTNTDVV DSFEEEQRKI 
61:	VYRGWKVMPF IIGNETFEKL GIIGTLSNLL VYLTSVFNLK SYTAATIINA FSGTINFGTF 
121:	IAAFLCDTYF GRYKTLSVAV IACFLGSFVI LLTAAIPSLH PVACGNKISC EGPSVGQILF 
181:	LLMGLGFLVV GAGGIRPCNL AFGADQFNPK SESGKKGINS FFNWYFFTFT FAQIISLTAV 
241:	VYIQSNVSWT IGLIIPVALM FLACVIFFAG DRLYVKVKAS GSPLAGIARV IAAAIKKRGL 
301:	KPVKQPWVNL YNHIPSNYAN TTLKYTDQFR FLDKAAIMTP EEKLNSDGTA SDPWKLCTLQ 
361:	QVEEVKCIVR VIPIWFASTI YYLAITIQMT YPVFQALQSD RRLGSGGFRI PAATYVVFLM 
421:	TGMTVFIIFY DRVLVPSLRR VTGLETGISL LQRIGAGFTF AIMSLLVSGF IEERRRNFAL 
481:	TKPTLGMAPR TGEISSMSAL WLIPQLTLAG IAEAFAAIGQ MEFYYKQFPE NMKSFAGSIF 
541:	YVGAGVSSYL ASFLISTVHR TTAHSPSGNW LAEDLNKAKL DYFYFMLTGL MVVNMAYFLL 
601:	MARWYRYKGG NDEDITEIET NEEETKQQQL QDKNSV