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2.A.18.2.1
General amino acid permease 1, AAP1 (transports most neutral and acidic amino acids but not aspartate or the basic amino acids)

Accession Number:Q42400
Protein Name:AAP1 aka F19C14.3 aka ZCF54
Length:485
Molecular Weight:52895.00
Species:Arabidopsis thaliana (Mouse-ear cress) [3702]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Amino acids

Cross database links:

RefSeq: NP_176132.1   
Entrez Gene ID: 842205   
Pfam: PF01490   
KEGG: ath:AT1G58360   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015175 F:neutral amino acid transmembrane transporte...
GO:0015293 F:symporter activity
GO:0043090 P:amino acid import
GO:0015804 P:neutral amino acid transport
GO:0009624 P:response to nematode

References (13)

[1] “Cloning a plant amino acid transporter by functional complementation of a yeast amino acid transport mutant.”  Hsu L.-C.et.al.   8356039
[2] “Expression cloning in yeast of a cDNA encoding a broad specificity amino acid permease from Arabidopsis thaliana.”  Frommer W.B.et.al.   8327465
[3] “Isolation and analysis of cDNA within a 300 kb Arabidopsis thaliana genomic region located around the 100 map unit of chromosome 1.”  Kato A.et.al.   10548732
[4] “Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.”  Theologis A.et.al.   11130712
[5] “Differential expression of two related amino acid transporters with differing substrate specificity in Arabidopsis thaliana.”  Kwart M.et.al.   8281191
[6] “Substrate specificity and expression profile of amino acid transporters (AAPs) in Arabidopsis.”  Fischer W.-N.et.al.   7608199
[7] “Kinetics and specificity of a H+/amino acid transporter from Arabidopsis thaliana.”  Boorer K.J.et.al.   8567681
[8] “Topology of NAT2, a prototypical example of a new family of amino acid transporters.”  Chang H.-C.et.al.   9374550
[9] “Developmental control of H+/amino acid permease gene expression during seed development of Arabidopsis.”  Hirner B.et.al.   9675899
[10] “High affinity amino acid transporters specifically expressed in xylem parenchyma and developing seeds of Arabidopsis.”  Okumoto S.et.al.   12244056
[11] “Low and high affinity amino acid H+-cotransporters for cellular import of neutral and charged amino acids.”  Fischer W.-N.et.al.   12148530
[12] “AAP1 transports uncharged amino acids into roots of Arabidopsis.”  Lee Y.-H.et.al.   17419840
[13] “AAP1 regulates import of amino acids into developing Arabidopsis embryos.”  Sanders A.et.al.   19392706

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
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FASTA formatted sequence
1:	MKSFNTEGHN HSTAESGDAY TVSDPTKNVD EDGREKRTGT WLTASAHIIT AVIGSGVLSL 
61:	AWAIAQLGWI AGTSILLIFS FITYFTSTML ADCYRAPDPV TGKRNYTYMD VVRSYLGGRK 
121:	VQLCGVAQYG NLIGVTVGYT ITASISLVAV GKSNCFHDKG HTADCTISNY PYMAVFGIIQ 
181:	VILSQIPNFH KLSFLSIMAA VMSFTYATIG IGLAIATVAG GKVGKTSMTG TAVGVDVTAA 
241:	QKIWRSFQAV GDIAFAYAYA TVLIEIQDTL RSSPAENKAM KRASLVGVST TTFFYILCGC 
301:	IGYAAFGNNA PGDFLTDFGF FEPFWLIDFA NACIAVHLIG AYQVFAQPIF QFVEKKCNRN 
361:	YPDNKFITSE YSVNVPFLGK FNISLFRLVW RTAYVVITTV VAMIFPFFNA ILGLIGAASF 
421:	WPLTVYFPVE MHIAQTKIKK YSARWIALKT MCYVCLIVSL LAAAGSIAGL ISSVKTYKPF 
481:	RTMHE