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2.A.18.5.3
The vacuolar GABA and glycine uptake transporter, VGAT. Also called "vesicular inhibitory amino acid transporter" (VIAAT); it is a 2Cl-/γ-aminobutyrate or glycine co-transporter in synaptic vesicles (Juge et al., 2009). GlyT2 and VIAAT cooperate to determine the vesicular glycinergic phenotype (Aubrey et al., 2007).

Accession Number:O35633
Protein Name:VGAT
Length:525
Molecular Weight:57381.00
Species:Mus musculus (Mouse) [10090]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cytoplasmic vesicle membrane1 / Multi-pass membrane protein2
Substrate Gamma-aminobutyric acid, Glycine

Cross database links:

Genevestigator: O35633
eggNOG: roNOG15425
HEGENOM: HBG713804
RefSeq: NP_033534.2   
Entrez Gene ID: 22348   
Pfam: PF01490   
KEGG: mmu:22348   

Gene Ontology

GO:0030659 C:cytoplasmic vesicle membrane
GO:0060077 C:inhibitory synapse
GO:0005887 C:integral to plasma membrane
GO:0015495 F:gamma-aminobutyric acid:hydrogen symporter ...
GO:0015187 F:glycine transmembrane transporter activity
GO:0015816 P:glycine transport
GO:0006836 P:neurotransmitter transport

References (5)

[1] “Cloning of a functional vesicular GABA and glycine transporter by screening of genome databases.”  Sagne C.et.al.   9395291
[2] “Mouse vesicular GABA transporter gene: genomic organization, transcriptional regulation and chromosomal localization.”  Ebihara S.et.al.   12573541
[3] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[4] “Cellular localization of the vesicular inhibitory amino acid transporter in the mouse and human retina.”  Jellali A.et.al.   12115694
[5] “Endogenously nitrated proteins in mouse brain: links to neurodegenerative disease.”  Sacksteder C.A.et.al.   16800626

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MATLLRSKLT NVATSVSNKS QAKVSGMFAR MGFQAATDEE AVGFAHCDDL DFEHRQGLQM 
61:	DILKSEGEPC GDEGAEAPVE GDIHYQRGGA PLPPSGSKDQ AVGAGGEFGG HDKPKITAWE 
121:	AGWNVTNAIQ GMFVLGLPYA ILHGGYLGLF LIIFAAVVCC YTGKILIACL YEENEDGEVV 
181:	RVRDSYVAIA NACCAPRFPT LGGRVVNVAQ IIELVMTCIL YVVVSGNLMY NSFPGLPVSQ 
241:	KSWSIIATAV LLPCAFLKNL KAVSKFSLLC TLAHFVINIL VIAYCLSRAR DWAWEKVKFY 
301:	IDVKKFPISI GIIVFSYTSQ IFLPSLEGNM QQPSEFHCMM NWTHIAACVL KGLFALVAYL 
361:	TWADETKEVI TDNLPGSIRA VVNLFLVAKA LLSYPLPFFA AVEVLEKSLF QEGSRAFFPA 
421:	CYGGDGRLKS WGLTLRCALV VFTLLMAIYV PHFALLMGLT GSLTGAGLCF LLPSLFHLRL 
481:	LWRKLLWHQV FFDVAIFVIG GICSVSGFVH SLEGLIEAYR TNAED