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2.A.18.7.2
Vacuolar amino acid transporter 4

Accession Number:P50944
Protein Name:Vacuolar amino acid transporter 4
Length:713
Molecular Weight:80026.00
Species:Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [559292]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Vacuole membrane1 / Multi-pass membrane protein2
Substrate Amino acids

Cross database links:

Genevestigator: P50944
eggNOG: COG0814
HEGENOM: HOG000185918
DIP: DIP-6754N
Entrez Gene ID: 855622   
Pfam: PF01490   
KEGG: sce:YNL101W   

Gene Ontology

GO:0000329 C:fungal-type vacuole membrane
GO:0016021 C:integral to membrane
GO:0015186 F:L-glutamine transmembrane transporter activity
GO:0015188 F:L-isoleucine transmembrane transporter activity
GO:0005302 F:L-tyrosine transmembrane transporter activity
GO:0032974 P:amino acid export from vacuole

References (6)

[1] “The sequence of a 21.3 kb DNA fragment from the left arm of yeast chromosome XIV reveals LEU4, MET4, POL1, RAS2, and six new open reading frames.”  Saiz J.E.et.al.   8701612
[2] “The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.”  Philippsen P.et.al.   9169873
[3] “A family of yeast proteins mediating bidirectional vacuolar amino acid transport.”  Russnak R.et.al.   11274162
[4] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258
[5] “Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry.”  Chi A.et.al.   17287358
[6] “A multidimensional chromatography technology for in-depth phosphoproteome analysis.”  Albuquerque C.P.et.al.   18407956

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MVTNNGDGEH LGIRRNGNLR HPSNNMKIPR RAQSTVLNSN PFYSRKYSMS TLTPRDICRS 
61:	VDSRVFVDMS SPNFQTLEDP HRDEIINSVR LNYLNSSKRS SVSHGNEAIP RVNPTKNSSA 
121:	STIAAANVDS DDDETNLSSA GGDITHDIYK LVKAEDPKRL RRPRSMENVT PKIEHHTKLS 
181:	SASGLNVPGG FRREFIVNKK RQEHQLNDSA SSDFTSHESD SINQSSPSSN QDIDKVPFLT 
241:	RNFLEFLYVF GHFAGESFED DFIPDSSNMM IRGEDERSAL LSRPDHMKVL PSAKGTTSTK 
301:	KVFLILLKSF IGTGVLFLPN AFHNGGLFFS VSMLAFFGIY SYWCYYILVQ AKSSCGVSSF 
361:	GDIGLKLYGP WMRIIILFSL VITQVGFSGA YMIFTAKNLQ AFLDNVFHVG VLPLSYLMVF 
421:	QTIIFIPLSF IRNISKLSLP SLLANFFIMA GLVIVIIFTA KRLFFDLMGT PAMGVVYGLN 
481:	ADRWTLFIGT AIFAFEGIGL IIPVQDSMRN PEKFPLVLAL VILTATILFI SIATLGYLAY 
541:	GSNVQTVILL NLPQSNIFVN LIQLFYSIAI MLSTPLQLFP AIKIIENKFF PKFTKIYVKH 
601:	DDLTTRVELR PNSGKLNWKI KWLKNFIRSI IVIIVVSIAY FGSDNLDKFV SVIGSLACIP 
661:	LVYIYPSMLH LRGNSLPETK GEFWRFKPML DTILIFFGIA SMLYTSYQSI FGV