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2.A.18.8.1
The electrogenic, proton-dependent amino acid:H+ symporter, PAT1 or LYAAT-1 (Slc36A1). Catalyzes uptake of L-Gly, L-Ala, L-Pro, γ-amino butyrate, and short chain D-amino acids such as proline and hydroxyproline with an aa/ H+ ratio of 1:1 (found in lysosomes) In humans, this is the iminoglycinuria protein (Boll et al., 2004Miyauchi et al., 2005; Broer, 2008). A disulfide bridge is essential for transport function (Dorn et al., 2009). Transports taurine and β-alanine by H+ symport with low affinity and high capacity across the intestinal brush boarder membrane (Anderson et al., 2009). Exhibits low affinity (Km= 1-10 mM) and transports amino acid-based drugs used to treat epilepsy, schizophrenia, bacterial infections, hyperglycemia and cancer (Thwaites and Anderson, 2011).

Accession Number:Q8K4D3
Protein Name:PAT1 aka mPAT1 aka SLC36A1
Length:475
Molecular Weight:52466.00
Species:Mus musculus (Mouse) [10090]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Amino acids, H+, Gamma-aminobutyric acid

Cross database links:

Genevestigator: Q8K4D3
HEGENOM: HBG444352
RefSeq: NP_694779.3   
Entrez Gene ID: 215335   
Pfam: PF01490   
KEGG: mmu:215335   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005765 C:lysosomal membrane
GO:0005886 C:plasma membrane
GO:0015187 F:glycine transmembrane transporter activity
GO:0015180 F:L-alanine transmembrane transporter activity
GO:0015193 F:L-proline transmembrane transporter activity
GO:0015293 F:symporter activity
GO:0015816 P:glycine transport
GO:0015808 P:L-alanine transport
GO:0015824 P:proline transport

References (2)

[1] “Functional characterization of two novel mammalian electrogenic proton-dependent amino acid cotransporters.”  Boll M.et.al.   11959859
[2] “Organization and expression of the SLC36 cluster of amino acid transporter genes.”  Bermingham J.R. Jr.et.al.   15058382

External Searches:

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  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSTQRLRNED YHDYSSTDVS PEESPSEGLG SFSPGSYQRL GENSSMTWFQ TLIHLLKGNI 
61:	GTGLLGLPLA VKNAGLLLGP LSLLVIGIVA VHCMGILVKC AHHLCRRLNK PFLDYGDTVM 
121:	YGLECSPSTW VRNHSHWGRR IVDFFLIVTQ LGFCCVYFVF LADNFKQVIE AANGTTTNCN 
181:	NNVTVIPTPT MDSRLYMLSF LPFLVLLSFI RNLRVLSIFS LLANISMFVS LIMIYQFIVQ 
241:	RIPDPSHLPL VAPWKTYPLF FGTAIFAFEG IGVVLPLENK MKDSQKFPLI LYLGMAIITV 
301:	LYISLGSLGY LQFGANIKGS ITLNLPNCWL YQSVKLLYSI GIFFTYALQF YVAAEIIIPA 
361:	IVSRVPEHFE LMVDLCVRTA MVCVTCVLAI LIPRLDLVIS LVGSVSSSAL ALIIPPLLEV 
421:	VTYYGEGISP LTVTKDALIS ILGFVGFVVG TYESLCELIQ PSHSDSSTNS TSAFI