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2.A.18.8.2
Electrogenic, proton-coupled, amino acid symporter 2 (PAT2; Tramdorin-1; SLC36A2) (transports small amino acids: glycine, alanine and proline; found in the ER, not in lysosomes, of neuronal cells in the brain and spinal cord; it can catalyze bidirectional transport depending on the driving force) (Boll et al., 2004Rubio-Aliaga et al., 2004). SLC36A2 is expressed at the apical surface of the human renal proximal tubule where it functions in the reabsorption of glycine, proline, hydroxyproline and amino acid derivatives with narrower substrate selectivity and higher affinity (Km 0.1-0.7 mM) than SLC36A1. Mutations in SLC36A2 lead to hyperglycinuria and iminoglycinuria.

Accession Number:Q8BHK3
Protein Name:PAT2
Length:478
Molecular Weight:52063.00
Species:Mus musculus (Mouse) [10090]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Amino acids, H+

Cross database links:

Genevestigator: Q8BHK3
eggNOG: roNOG05057
HEGENOM: HBG444352
RefSeq: NP_694810.2   
Entrez Gene ID: 246049   
Pfam: PF01490   
KEGG: mmu:246049   

Gene Ontology

GO:0005737 C:cytoplasm
GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005280 F:hydrogen:amino acid symporter activity
GO:0015180 F:L-alanine transmembrane transporter activity
GO:0015193 F:L-proline transmembrane transporter activity
GO:0015816 P:glycine transport
GO:0015808 P:L-alanine transport
GO:0015824 P:proline transport
GO:0015992 P:proton transport

References (9)

[1] “Functional characterization of two novel mammalian electrogenic proton-dependent amino acid cotransporters.”  Boll M.et.al.   11959859
[2] “Identification of genes that are downregulated in the absence of the POU domain transcription factor pou3f1 (Oct-6, Tst-1, SCIP) in sciatic nerve.”  Bermingham J.R. Jr.et.al.   12451123
[3] “The transcriptional landscape of the mammalian genome.”  Carninci P.et.al.   16141072
[4] “Lineage-specific biology revealed by a finished genome assembly of the mouse.”  Church D.M.et.al.   19468303
[5] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[6] “A cluster of proton/amino acid transporter genes in the human and mouse genomes.”  Boll M.et.al.   12809675
[7] “Substrate specificity and transport mode of the proton-dependent amino acid transporter mPAT2.”  Foltz M.et.al.   15291811
[8] “The proton/amino acid cotransporter PAT2 is expressed in neurons with a different subcellular localization than its paralog PAT1.”  Rubio-Aliaga I.et.al.   14600155
[9] “Organization and expression of the SLC36 cluster of amino acid transporter genes.”  Bermingham J.R. Jr.et.al.   15058382

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSVTKSARSP QVATPLNLDL PESAKKLQSQ DPSPANGSSS ESSKKTKGIT GFQTLVHLVK 
61:	GNMGTGILGL PLAVKNAGIL MGPLSLLVMG LIACHCMHIL VRCAQRFCHR LNKPFMDYGD 
121:	TVMHGLAFSP NAWLQNHAHW GRRVVSFFLI VTQLGFCCVY IVFLADNLKQ VVEAVNSTTI 
181:	SCHKNETVVL TPTMDSRLYM LSFLPVLGLL VFVRNLRVLT IFSLLANISM LVSLVIIAQY 
241:	IIQEIPDASQ LPLVASWKTY PLFFGTAIFS FESIGVVLPL ENKMKDARGF PTILSLGMSI 
301:	ITTLYIAIGA LGYLRFGDDI KASITLNLPN CWLYQSVKLL YVVGILCTYA LQFYVPAEII 
361:	IPLAVSQVSK RWALPVDLSI RLALVCLTCM LAILIPRLDL VLSLVGSVSS SALALIIPPL 
421:	LEVVTYYGEG ISPLTVTKDA LISILGFMGF VVGTYQALDE LIKSGNSPAL SNSTMFIQ