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2.A.19.1.1
Ca2+:H+ antiporter (also catalyzes Na+:H+ and K+:H+ antiport in processes that have been shown to be physiologically important under certain conditions) (Ivey et al., 1993; Radchenko et al., 2006)

Accession Number:P31801
Protein Name:ChaA aka B1216
Length:366
Molecular Weight:39168.00
Species:Escherichia coli [83333]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate K+, Ca2+, Na+, H+

Cross database links:

Genevestigator: P31801
EchoBASE: EB1703
EcoGene: EG11753
eggNOG: COG0387
HEGENOM: HBG575576
RefSeq: AP_001844.1    NP_415734.1   
Entrez Gene ID: 945790   
Pfam: PF01699   
BioCyc: EcoCyc:CHAA-MONOMER    ECOL168927:B1216-MONOMER   
KEGG: ecj:JW1207    eco:b1216   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015386 F:potassium:hydrogen antiporter activity
GO:0015385 F:sodium:hydrogen antiporter activity
GO:0006816 P:calcium ion transport
GO:0030007 P:cellular potassium ion homeostasis
GO:0006883 P:cellular sodium ion homeostasis
GO:0006813 P:potassium ion transport
GO:0015992 P:proton transport
GO:0051481 P:reduction of cytosolic calcium ion concentr...
GO:0006814 P:sodium ion transport
GO:0055085 P:transmembrane transport

References (5)

[1] “Cloning and characterization of a putative Ca2+/H+ antiporter gene from Escherichia coli upon functional complementation of Na+/H+ antiporter-deficient strains by the overexpressed gene.”  Ivey D.M.et.al.   8496184
[2] “A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.”  Oshima T.et.al.   8905232
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSNAQEAVKT RHKETSLIFP VLALVVLFLW GSSQTLPVVI AINLLALIGI LSSAFSVVRH 
61:	ADVLAHRLGE PYGSLILSLS VVILEVSLIS ALMATGDAAP TLMRDTLYSI IMIVTGGLVG 
121:	FSLLLGGRKF ATQYMNLFGI KQYLIALFPL AIIVLVFPMA LPAANFSTGQ ALLVALISAA 
181:	MYGVFLLIQT KTHQSLFVYE HEDDSDDDDP HHGKPSAHSS LWHAIWLIIH LIAVIAVTKM 
241:	NASSLETLLD SMNAPVAFTG FLVALLILSP EGLGALKAVL NNQVQRAMNL FFGSVLATIS 
301:	LTVPVVTLIA FMTGNELQFA LGAPEMVVMV ASLVLCHISF STGRTNVLNG AAHLALFAAY 
361:	LMTIFA