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2.A.19.2.3
High affinity vacuolar (tonoplast) Ca2+:H+ antiporter (also exports Cd2+ and Zn2+; Shigaki et al., 2005) expressed in leaves (Cheng et al., 2005). (Determines sensitivity to abscisic acid and sugar during germination and tolerance to ethylene during early seedling development (Zhao et al., 2008))

Accession Number:Q39253
Protein Name:Cax1
Length:463
Molecular Weight:50177.00
Species:Arabidopsis thaliana (Mouse-ear cress) [3702]
Number of TMSs:10
Location1 / Topology2 / Orientation3: Vacuole membrane1 / Multi-pass membrane protein2
Substrate Ca2+, Cd2+, Zn2+, H+

Cross database links:

Genevestigator: Q39253
eggNOG: KOG1397
HEGENOM: HBG738140
RefSeq: NP_181352.1   
Entrez Gene ID: 818395   
Pfam: PF01699   
KEGG: ath:AT2G38170   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0009705 C:plant-type vacuole membrane
GO:0015085 F:calcium ion transmembrane transporter activity
GO:0015369 F:calcium:hydrogen antiporter activity
GO:0005515 F:protein binding
GO:0006816 P:calcium ion transport
GO:0030026 P:cellular manganese ion homeostasis
GO:0006882 P:cellular zinc ion homeostasis
GO:0009631 P:cold acclimation
GO:0009651 P:response to salt stress
GO:0055085 P:transmembrane transport

References (16)

[1] “Regulation of CAX1, an Arabidopsis Ca(2+)/H+ antiporter. Identification of an N-terminal autoinhibitory domain.”  Pittman J.K.et.al.   11706183
[2] “Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.”  Lin X.et.al.   10617197
[3] “Empirical analysis of transcriptional activity in the Arabidopsis genome.”  Yamada K.et.al.   14593172
[4] “CAX1, an H+/Ca2+ antiporter from Arabidopsis.”  Hirschi K.D.et.al.   8710949
[5] “Expression of Arabidopsis CAX1 in tobacco: altered calcium homeostasis and increased stress sensitivity.”  Hirschi K.D.et.al.   10559438
[6] “Structural determinants of Ca2+ transport in the Arabidopsis H+/Ca2+ antiporter CAX1.”  Shigaki T.et.al.   11562366
[7] “Mechanism of N-terminal autoinhibition in the Arabidopsis Ca(2+)/H(+) antiporter CAX1.”  Pittman J.K.et.al.   12006570
[8] “Cloning and characterization of CXIP1 A novel PICOT domain-containing Arabidopsis protein that associates with CAX1.”  Cheng N.-H.et.al.   12480930
[9] “The Arabidopsis cax1 mutant exhibits impaired ion homeostasis, development, and hormonal responses and reveals interplay among vacuolar transporters.”  Cheng N.-H.et.al.   12566577
[10] “Mutations in the Ca2+/H+ transporter CAX1 increase CBF/DREB1 expression and the cold-acclimation response in Arabidopsis.”  Catala R.et.al.   14630965
[11] “Characterization of CXIP4, a novel Arabidopsis protein that activates the H+/Ca2+ antiporter, CAX1.”  Cheng N.-H.et.al.   14960315
[12] “Genetic manipulation for enhancing calcium content in potato tuber.”  Park S.et.al.   15998121
[13] “Evidence of differential pH regulation of the Arabidopsis vacuolar Ca2+/H+ antiporters CAX1 and CAX2.”  Pittman J.K.et.al.   15862304
[14] “Identification of a crucial histidine involved in metal transport activity in the Arabidopsis cation/H+ exchanger CAX1.”  Shigaki T.et.al.   15994298
[15] “Functional association of Arabidopsis CAX1 and CAX3 is required for normal growth and ion homeostasis.”  Cheng N.-H.et.al.   16055687
[16] “Increased calcium levels and prolonged shelf life in tomatoes expressing Arabidopsis H+/Ca2+ transporters.”  Park S.et.al.   16244156

External Searches:

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MAGIVTEPWS VAENGNPSIT AKGSSRELRL GRTAHNMSSS SLRKKSDLRV IQKVPYKGLK 
61:	DFLSNLQEVI LGTKLAILFP AIPAAIICTY CGVSQPWIFG LSLLGLTPLA ERVSFLTEQL 
121:	AFYTGPTLGG LLNATCGNAT ELIIAILALT NNKVAVVKYS LLGSILSNLL LVLGTSLFCG 
181:	GIANIRREQR FDRKQADVNF FLLLLGFLCH LLPLLVGYLK NGEASAAVLS DMQLSISRGF 
241:	SIVMLISYIA YLVFQLWTHR QLFDAQEQED EYDDDVEQET AVISFWSGFA WLVGMTLVIA 
301:	LLSEYVVATI EEASDKWNLS VSFISIILLP IVGNAAEHAG AVIFAFKNKL DISLGVALGS 
361:	ATQIGLFVVP LTIIVAWILG INMDLNFGPL ETGCLAVSII ITAFTLQDGS SHYMKGLVLL 
421:	LCYFIIAICF FVDKLPQKQN AIHLGHQAMN NVVTATGGGV FSS