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2.A.19.3.1
The 10 TMS cardiac Ca2+:3 Na+ antiporter, NCX1 (Ren and Philipson 2013).  The Ca2+ sensor (residues 371-508) binds cytoplasmic Ca2+ allosterically to activate exchange activity) (Nicoll et al., 2006; Ren et al., 2006) NCX1 forms homodimers (Ren et al., 2008). It is present in mitochondria where it catalyzes Ca2+ efflux. TMS packing has been analyzed by Ren et al. (2010). Cytoplasmic Ca2+ regulates the dimeric NCX by binding to two adjacent Ca2+-binding domains (CBD1 and CBD2) located in the large intracellular loop between transmembrane segments 5 and 6. John et al. (2011) showed that Ca2+decreases the distance between the cytoplasmic loops of NCX pairs, thereby activating transport.  Ser110 in TMS2 plays a role in both Na+ and Ca2+ transport (Ottolia and Philipson 2013).

Accession Number:P48765
Protein Name:NAC1 aka SLC8A1
Length:970
Molecular Weight:108027.00
Species:Bos taurus (Bovine) [9913]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Ca2+, Na+

Cross database links:

eggNOG: maNOG07564
RefSeq: NP_788805.1   
Entrez Gene ID: 337925   
Pfam: PF03160    PF01699   
KEGG: bta:337925   

Gene Ontology

GO:0005901 C:caveola
GO:0016021 C:integral to membrane
GO:0005432 F:calcium:sodium antiporter activity
GO:0005516 F:calmodulin binding
GO:0031072 F:heat shock protein binding
GO:0006816 P:calcium ion transport
GO:0007154 P:cell communication
GO:0007204 P:elevation of cytosolic calcium ion concentr...
GO:0051924 P:regulation of calcium ion transport
GO:0002028 P:regulation of sodium ion transport
GO:0002026 P:regulation of the force of heart contraction
GO:0006814 P:sodium ion transport
GO:0055085 P:transmembrane transport

References (3)

[1] “Cloning and expression of the bovine cardiac sodium-calcium exchanger.”  Aceto J.F.et.al.   1416984
[2] “Purification and amino-terminal sequence of the bovine cardiac sodium-calcium exchanger: evidence for the presence of a signal sequence.”  Durkin J.T.et.al.   1929404
[3] “Identification of the sodium-calcium exchanger as the major ricin-binding glycoprotein of bovine rod outer segments and its localization to the plasma membrane.”  Reid D.M.et.al.   2334719

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MLQFSLSPTL SMGFHVIAMV ALLFSHVDHI SAETEMEGEG NETGECTGSY YCKKGVILPI 
61:	WEPQDPSFGD KIARATVYFV AMVYMFLGVS IIADRFMSSI EVITSQEKEI TIKKPNGETT 
121:	KTTVRIWNET VSNLTLMALG SSAPEILLSV IEVCGHNFTA GDLGPSTIVG SAAFNMFIII 
181:	ALCVYVVPDG ETRKIKHLRV FFVTAAWSIF AYTWLYIILS VSSPGVVEVW EGLLTFFFFP 
241:	ICVVFAWVAD RRLLFYKYVY KRYRAGKQRG MIIEHEGDRP SSKTEIEMDG KVVNSHVDSF 
301:	LDGALVLEVD ERDQDDEEAR REMARILKEL KQKHPEKEIE QLIELANYQV LSQQQKSRAF 
361:	YRIQATRLMT GAGNILKRHA ADQARKAVSM HEVNTEVAEN DPVSKIFFEQ GTYQCLENCG 
421:	TVALTIIRRG GDLTNTVFVD FRTEDGTANA GSDYEFTEGT VVFKPGETQK EIRVGIIDDD 
481:	IFEEDENFLV HLSNVKVSLE ASEDGILEAS HVSTLACLGS PSTATVTIFD DDHAGIFTFE 
541:	EPVTHVSESI GIMEVKVLRT SGARGNVIVP YKTIEGTARG GGEDFEDTCG ELEFQNDEIV 
601:	KTISVKVIDD EEYEKNKTFF LEIGEPRLVE MSEKKALLLN ELGGFTITGK YLYGQPVFRK 
661:	VHAREHPLPS TIITIADEYD DKQPLTSKEE EERRIAEMGR PILGEHTRLE VIIEESYEFK 
721:	STVDKLIKKT NLALVVGTNS WREQFIEAIT VSAGEDDDDD ECGEEKLPSC FDYVMHFLTV 
781:	FWKVLFAFVP PTEYWNGWAC FIVSILMIGL LTAFIGDLAS HFACTIALKD SVTAVVFVAL 
841:	GTSVPDTFAS KVAATQDQYA DASIGNVTGS NAVNVFLGIG VAWSIAAIYH AANGEQFKVS 
901:	PGTLAFSVTL FTIFAFINVG VLLYRRRPEI GGELGGPRTA KLLTSCLFVL LWLLYIFFSS 
961:	LEAYCHIKGF