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2.A.19.4.5
The K+-dependent Na+/Ca2+ exchanger, MCKX4 (has 40x higher affinity for K+ than NCKX2 due to a threonine to alanine substitution at position 551 in NCKX2 (Visser et al., 2007)). NCKX4 is highly expressed and regulates Ca2+ transport in ameloblasts during amelogenesis (the formation of tooth enamel). In fact, MCKX4 is critical for enamel maturation (Wang et al. 2014). Residues involved in Na+ binding have been identified (Altimimi et al. 2010).

Accession Number:Q8NFF2
Protein Name:NCKX4
Length:605
Molecular Weight:66951.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Ca2+, K+, Na+

Cross database links:

Genevestigator: Q8NFF2 Q8NFF2
eggNOG: prNOG12507 COG0530
HEGENOM: HBG444187
RefSeq: NP_705932.1    NP_705933.1    NP_705934.1   
Entrez Gene ID: 123041   
Pfam: PF01699   
OMIM: 210750  phenotype
609840  gene
KEGG: hsa:123041    hsa:123041   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0015297 F:antiporter activity
GO:0015293 F:symporter activity
GO:0006816 P:calcium ion transport
GO:0006813 P:potassium ion transport
GO:0006814 P:sodium ion transport
GO:0055085 P:transmembrane transport
GO:0005886 C:plasma membrane
GO:0006811 P:ion transport

References (11)

[1] “Molecular cloning of a fourth member of the potassium-dependent sodium-calcium exchanger gene family, NCKX4.”  Li X.-F.et.al.   12379639
[2] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[3] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[4] “The full-ORF clone resource of the German cDNA consortium.”  Bechtel S.et.al.   17974005
[5] “Genetic determinants of hair, eye and skin pigmentation in Europeans.”  Sulem P.et.al.   17952075
[6] “Molecular cloning of a fourth member of the potassium-dependent sodium-calcium exchanger gene family, NCKX4.”  Li X.-F.et.al.   12379639
[7] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[8] “The DNA sequence and analysis of human chromosome 14.”  Heilig R.et.al.   12508121
[9] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[10] “The full-ORF clone resource of the German cDNA consortium.”  Bechtel S.et.al.   17974005
[11] “Genetic determinants of hair, eye and skin pigmentation in Europeans.”  Sulem P.et.al.   17952075

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MLPQQVGFVC AVLALVCCAS GLFGSLGHKT ASASKRVLPD TWRNRKLMAP VNGTQTAKNC 
61:	TDPAIHEFPT DLFSNKERQH GAVLLHILGA LYMFYALAIV CDDFFVPSLE KICERLHLSE 
121:	DVAGATFMAA GSSTPELFAS VIGVFITHGD VGVGTIVGSA VFNILCIIGV CGLFAGQVVR 
181:	LTWWAVCRDS VYYTISVIVL IVFIYDEQIV WWEGLVLIIL YVFYILIMKY NVKMQAFFTV 
241:	KQKSIANGNP VNSELEAGND FYDGSYDDPS VPLLGQVKEK PQYGKNPVVM VDEIMSSSPP 
301:	KFTFPEAGLR IMITNKFGPR TRLRMASRII INERQRLINS ANGVSSKPLQ NGRHENIENG 
361:	NVPVENPEDP QQNQEQQPPP QPPPPEPEPV EADFLSPFSV PEARGDKVKW VFTWPLIFLL 
421:	CVTIPNCSKP RWEKFFMVTF ITATLWIAVF SYIMVWLVTI IGYTLGIPDV IMGITFLAAG 
481:	TSVPDCMASL IVARQGLGDM AVSNTIGSNV FDILVGLGVP WGLQTMVVNY GSTVKINSRG 
541:	LVYSVVLLLG SVALTVLGIH LNKWRLDRKL GVYVLVLYAI FLCFSIMIEF NVFTFVNLPM 
601:	CREDD