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2.A.19.5.3
Na+:Ca2+ exchanger, NCX_Mj (3-d structure known at 1.9 Å resolution; PDB# 3V5U (Liao et al., 2012). Contains 10 TMSs with two 5 TMS repeats. Four ion binding sites near the center of the protein are present, one specific for Ca2+ and three probably for Na+. Two passageways allow for Na+ and Ca2+ access from the external side.  However see a more recent analysis reported for 2.A.19.8.2 (Nishizawa et al. 2013).  Transport of both Na+ and Ca2+ requires protonation of D240, but this side chain does not coordinate either ion, implying that the ion exchange stoichiometry is 3:1 and that translocation of Na+ across the membrane is electrogenic although transport of Ca2+ is not (Marinelli et al. 2014).

Accession Number:Q57556
Protein Name:Uncharacterized membrane protein MJ0091
Length:302
Molecular Weight:32213.00
Species:Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) [243232]
Number of TMSs:10
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Ca2+, Na+

Cross database links:

eggNOG: COG0530
HEGENOM: HBG538237
Entrez Gene ID: 1450930   
Pfam: PF01699   
KEGG: mja:MJ_0091   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0055085 P:transmembrane transport

References (1)

[1] “Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.”  Bult C.J.et.al.   8688087
Structure:
3V5S   3V5U   5hwx   5hxc   5hxh   5jdf     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MLILGVGYFL LGLILLYYGS DWFVLGSERI ARHFNVSNFV IGATVMAIGT SLPEILTSAY 
61:	ASYMHAPGIS IGNAIGSCIC NIGLVLGLSA IISPIIVDKN LQKNILVYLL FVIFAAVIGI 
121:	DGFSWIDGVV LLILFIIYLR WTVKNGSAEI EENNDKNNPS VVFSLVLLII GLIGVLVGAE 
181:	LFVDGAKKIA LALDISDKVI GFTLVAFGTS LPELMVSLAA AKRNLGGMVL GNVIGSNIAD 
241:	IGGALAVGSL FMHLPAENVQ MAVLVIMSLL LYLFAKYSKI GRWQGILFLA LYIIAIASLR 
301:	MG