TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


2.A.19.7.1
Low affinity vacuolar monovalent cation (Na+ (Km=20 mM) or K+(Km=80 mM)):H+ antiporter, Vnx1. (Ca2+ is not transported; plays roles in ion and pH homeostasis) (Cagnac et al., 2007)

Accession Number:P42839
Protein Name:Putative cation exchanger aka Vnx1
Length:908
Molecular Weight:102499.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:13
Location1 / Topology2 / Orientation3: Vacuole membrane1 / Multi-pass membrane protein2
Substrate Na+, K+, H+

Cross database links:

Genevestigator: P42839
eggNOG: fuNOG05616
HEGENOM: HBG398302
DIP: DIP-4268N
RefSeq: NP_014078.1   
Entrez Gene ID: 855395   
Pfam: PF03733    PF01699   
KEGG: sce:YNL321W   

Gene Ontology

GO:0000324 C:fungal-type vacuole
GO:0016021 C:integral to membrane
GO:0005774 C:vacuolar membrane
GO:0015386 F:potassium:hydrogen antiporter activity
GO:0015385 F:sodium:hydrogen antiporter activity
GO:0006813 P:potassium ion transport
GO:0006814 P:sodium ion transport
GO:0055085 P:transmembrane transport

References (10)

[1] “Sequencing analysis of a 24.7 kb fragment of yeast chromosome XIV identifies six known genes, a new member of the hexose transporter family and ten new open reading frames.”  Maftahi M.et.al.   7502583
[2] “The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.”  Philippsen P.et.al.   9169873
[3] “Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae.”  Ficarro S.B.et.al.   11875433
[4] “Global analysis of protein expression in yeast.”  Ghaemmaghami S.et.al.   14562106
[5] “A proteomics approach to understanding protein ubiquitination.”  Peng J.et.al.   12872131
[6] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258
[7] “Identification and characterization of Vnx1p, a novel type of vacuolar monovalent cation/H+ antiporter of Saccharomyces cerevisiae.”  Cagnac O.et.al.   17588950
[8] “Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.”  Li X.et.al.   17330950
[9] “Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases.”  Smolka M.B.et.al.   17563356
[10] “A multidimensional chromatography technology for in-depth phosphoproteome analysis.”  Albuquerque C.P.et.al.   18407956

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MAKNNHISAS GNSTSGDHRL KEEVLTPTTS ASTPHRIFSV DDDPKEIQND IRYLEGLHEG 
61:	LKFALHANKS KRSVSSQSPI VHSSNNTLHH HEHQQHLPPT LESLSSKSHS VPDLNTATPS 
121:	SPKRMHSSIR ELPHDDNDDE DANDDSRFII HDSHGHDLLI DEINCQSPSH LENNDQASNA 
181:	SSTESFTLRE RQDAINETHP FGIRIWKPAL YKKHRSVQRT AAQDIHETQL KTITWEVTCS 
241:	NVLWFILFGF PIAILFYSAA IVVFLLGGGG LVTNSAKEYS KCLYKLANYF LWPFGKMVYL 
301:	LQDEQYLQED KDEGISMQQF YNWVTSYSNR LVFHQSQAKF QQREDHPAPA TESSSLMPPA 
361:	NTTATPLNSN HPSYNSIRHE IPHAAAQRRY FGRGKWSWGR VLFYTIFHLV LQPILAVLSL 
421:	CLWLLVFTIP MSNVLWQIMY HCRRHPLALG FKYVENSSQS HENEITQQQL NKNILLCTFR 
481:	AAGWHYYKYT VDGTNVIVVN LISIVFFTIF DFYVLKNFLH WKTWFTYESS IFILCLTSTI 
541:	PLAFYIGQAV ASISAQTSMG VGAVINAFFS TIVEIFLYCV ALQQKKGLLV EGSMIGSILG 
601:	AVLLLPGLSM CGGALNRKTQ RYNPASAGVS SALLIFSMIV MFVPTVLYEI YGGYSVNCAD 
661:	GANDRDCTFS HPPLKFNRLF THVIQPMSIS CAIVLFCAYI IGLWFTLRTH AKMIWQLPIA 
721:	DPTSTAPEQQ EQNSHDAPNW SRSKSTCILL MSTLLYAIIA EILVSCVDAV LEDIPSLNPK 
781:	FLGLTIFALI PNTTEFLNAI SFAIHGNVAL SMEIGSAYAL QVCLLQIPSL VIYSIFYTWN 
841:	VKKSMINIRT QMFPLVFPRW DIFGAMTSVF MFTYLYAEGK SNYFKGSMLI LLYIIIVVGF 
901:	YFQGALSE