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2.A.20.1.1
Low affinity Pi or phosphate-zinc complex uptake transporter #1, PitA (Km=2 μM) (Me·Pi:H+ symporter) (Beard et al. 2000; Jackson et al. 2008). Also transports tellurite (TeO32-) slowly (Elías et al. 2012; Borghese et al. 2016).

Accession Number:P0AFJ7
Protein Name:Low-affinity inorganic phosphate transporter 1 PitA aka PIT aka B3493
Length:499
Molecular Weight:53389.00
Species:Escherichia coli [83333]
Number of TMSs:10
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate phosphate, Phosphate-zinc, H+, Tellurite

Cross database links:

Genevestigator: P0AFJ7
EchoBASE: EB2142
EcoGene: EG12230
eggNOG: COG0306
HEGENOM: HBG681698
RefSeq: AP_004300.1    NP_417950.1   
Entrez Gene ID: 948009   
Pfam: PF01384   
BioCyc: EcoCyc:PITA-MONOMER    ECOL168927:B3493-MONOMER   
KEGG: ecj:JW3460    eco:b3493   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005315 F:inorganic phosphate transmembrane transport...
GO:0006817 P:phosphate transport

References (4)

[1] “Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes.”  Sofia H.J.et.al.   8041620
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MLHLFAGLDL HTGLLLLLAL AFVLFYEAIN GFHDTANAVA TVIYTRAMRS QLAVVMAAVF 
61:	NFLGVLLGGL SVAYAIVHML PTDLLLNMGS SHGLAMVFSM LLAAIIWNLG TWYFGLPASS 
121:	SHTLIGAIIG IGLTNALMTG TSVVDALNIP KVLSIFGSLI VSPIVGLVFA GGLIFLLRRY 
181:	WSGTKKRARI HLTPAEREKK DGKKKPPFWT RIALILSAIG VAFSHGANDG QKGIGLVMLV 
241:	LIGVAPAGFV VNMNATGYEI TRTRDAINNV EAYFEQHPAL LKQATGADQL VPAPEAGATQ 
301:	PAEFHCHPSN TINALNRLKG MLTTDVESYD KLSLDQRSQM RRIMLCVSDT IDKVVKMPGV 
361:	SADDQRLLKK LKSDMLSTIE YAPVWIIMAV ALALGIGTMI GWRRVATTIG EKIGKKGMTY 
421:	AQGMSAQMTA AVSIGLASYT GMPVSTTHVL SSSVAGTMVV DGGGLQRKTV TSILMAWVFT 
481:	LPAAVLLSGG LYWLSLQFL