TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


2.A.20.1.2
Low affinity Pi transporter #2, PitB (Km=30 μM) PitB, like PitA is also a Me·Pi:H+ symporter (Borghese et al. 2016)

Accession Number:P43676
Protein Name:PitB aka B2987
Length:499
Molecular Weight:53809.00
Species:Escherichia coli [83333]
Number of TMSs:10
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate phosphate

Cross database links:

Genevestigator: P43676
EchoBASE: EB2721
EcoGene: EG12883
eggNOG: COG0306
HEGENOM: HBG681698
RefSeq: AP_003542.1    NP_417461.1   
Entrez Gene ID: 947475   
Pfam: PF01384   
BioCyc: EcoCyc:PITB-MONOMER    ECOL168927:B2987-MONOMER   
KEGG: ecj:JW2955    eco:b2987   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005315 F:inorganic phosphate transmembrane transport...
GO:0006817 P:phosphate transport

References (4)

[1] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[2] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[3] “Glutathionylspermidine metabolism in Escherichia coli. Purification, cloning, overproduction, and characterization of a bifunctional glutathionylspermidine synthetase/amidase.”  Bollinger J.M. Jr.et.al.   7775463
[4] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MLNLFVGLDI YTGLLLLLAL AFVLFYEAIN GFHDTANAVA AVIYTRAMQP QLAVVMAAFF 
61:	NFFGVLLGGL SVAYAIVHML PTDLLLNMGS THGLAMVFSM LLAAIIWNLG TWFFGLPASS 
121:	SHTLIGAIIG IGLTNALLTG SSVMDALNLR EVTKIFSSLI VSPIVGLVIA GGLIFLLRRY 
181:	WSGTKKRDRI HRIPEDRKKK KGKRKPPFWT RIALIVSAAG VAFSHGANDG QKGIGLVMLV 
241:	LVGIAPAGFV VNMNASGYEI TRTRDAVTNF EHYLQQHPEL PQKLIAMEPP LPAASTDGTQ 
301:	VTEFHCHPAN TFDAIARVKT MLPGNMESYE PLSVSQRSQL RRIMLCISDT SAKLAKLPGV 
361:	SKEDQNLLKK LRSDMLSTIE YAPVWIIMAV ALALGIGTMI GWRRVAMTIG EKIGKRGMTY 
421:	AQGMAAQMTA AVSIGLASYI GMPVSTTHVL SSAVAGTMVV DGGGLQRKTV TSILMAWVFT 
481:	LPAAIFLSGG LYWIALQLI