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2.A.20.2.7
Na+-dependent phosphate transporter 1 (PiT-1). Gibbon ape leukemia virus receptor 1 (GLVR-1), Slc20A1 (KM=24μM).  The protein has an experimentally tested 12 TMS topology (Farrell et al. 2009).  A knock-out mutation of the mouse orthologue results in abnormal endocytosis in the yolk sac visceral endoderm and embyonic death at the 12.5 day stage (Wallingford and Giachelli 2014).

Accession Number:Q8WUM9
Protein Name:Sodium-dependent phosphate transporter 1
Length:679
Molecular Weight:73700.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate phosphate, Na+

Cross database links:

Genevestigator: Q8WUM9 Q8WUM9
eggNOG: prNOG08513 COG0306
HEGENOM: HBG681698 HOG000231892
Entrez Gene ID: 6574   
Pfam: PF01384   
KEGG: hsa:6574    hsa:6574   

Gene Ontology

GO:0005887 C:integral to plasma membrane
GO:0005315 F:inorganic phosphate transmembrane transporter activity
GO:0004872 F:receptor activity
GO:0004871 F:signal transducer activity
GO:0006796 P:phosphate-containing compound metabolic process
GO:0043123 P:positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0005316 F:high affinity inorganic phosphate:sodium symporter activity
GO:0005436 F:sodium:phosphate symporter activity

References (30)

[1] “Characterization of a human gene conferring sensitivity to infection by gibbon ape leukemia virus.”  O'Hara B.et.al.   2078500
[2] “Generation and annotation of the DNA sequences of human chromosomes 2 and 4.”  Hillier L.W.et.al.   15815621
[3] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[4] “Characterization of the human Glvr-1 phosphate transporter/retrovirus receptor gene and promoter region.”  Palmer G.et.al.   9889306
[5] “Feline leukemia virus subgroup B uses the same cell surface receptor as gibbon ape leukemia virus.”  Takeuchi Y.et.al.   1309898
[6] “GLVR1, a receptor for gibbon ape leukemia virus, is homologous to a phosphate permease of Neurospora crassa and is expressed at high levels in the brain and thymus.”  Johann S.V.et.al.   1531369
[7] “Definition of a domain of GLVR1 which is necessary for infection by gibbon ape leukemia virus and which is highly polymorphic between species.”  Johann S.V.et.al.   8411375
[8] “The cellular receptor for gibbon ape leukemia virus is a novel high affinity sodium-dependent phosphate transporter.”  Olah Z.et.al.   7929240
[9] “A family of retroviruses that utilize related phosphate transporters for cell entry.”  Miller D.G.et.al.   7966619
[10] “Cell-surface receptors for gibbon ape leukemia virus and amphotropic murine retrovirus are inducible sodium-dependent phosphate symporters.”  Kavanaugh M.P.et.al.   8041748
[11] “Phosphate regulation of vascular smooth muscle cell calcification.”  Jono S.et.al.   11009570
[12] “Reassessing the role of region A in Pit1-mediated viral entry.”  Farrell K.B.et.al.   12097582
[13] “Role of interleukin-8 in PiT-1 expression and CXCR1-mediated inorganic phosphate uptake in chondrocytes.”  Cecil D.L.et.al.   15641067
[14] “A quantitative atlas of mitotic phosphorylation.”  Dephoure N.et.al.   18669648
[15] “Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.”  Gauci S.et.al.   19413330
[16] “Characterization of a human gene conferring sensitivity to infection by gibbon ape leukemia virus.”  O'Hara B.et.al.   2078500
[17] “Generation and annotation of the DNA sequences of human chromosomes 2 and 4.”  Hillier L.W.et.al.   15815621
[18] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[19] “Characterization of the human Glvr-1 phosphate transporter/retrovirus receptor gene and promoter region.”  Palmer G.et.al.   9889306
[20] “Feline leukemia virus subgroup B uses the same cell surface receptor as gibbon ape leukemia virus.”  Takeuchi Y.et.al.   1309898
[21] “GLVR1, a receptor for gibbon ape leukemia virus, is homologous to a phosphate permease of Neurospora crassa and is expressed at high levels in the brain and thymus.”  Johann S.V.et.al.   1531369
[22] “Definition of a domain of GLVR1 which is necessary for infection by gibbon ape leukemia virus and which is highly polymorphic between species.”  Johann S.V.et.al.   8411375
[23] “The cellular receptor for gibbon ape leukemia virus is a novel high affinity sodium-dependent phosphate transporter.”  Olah Z.et.al.   7929240
[24] “A family of retroviruses that utilize related phosphate transporters for cell entry.”  Miller D.G.et.al.   7966619
[25] “Cell-surface receptors for gibbon ape leukemia virus and amphotropic murine retrovirus are inducible sodium-dependent phosphate symporters.”  Kavanaugh M.P.et.al.   8041748
[26] “Phosphate regulation of vascular smooth muscle cell calcification.”  Jono S.et.al.   11009570
[27] “Reassessing the role of region A in Pit1-mediated viral entry.”  Farrell K.B.et.al.   12097582
[28] “Role of interleukin-8 in PiT-1 expression and CXCR1-mediated inorganic phosphate uptake in chondrocytes.”  Cecil D.L.et.al.   15641067
[29] “A quantitative atlas of mitotic phosphorylation.”  Dephoure N.et.al.   18669648
[30] “Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.”  Gauci S.et.al.   19413330

External Searches:

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MATLITSTTA ATAASGPLVD YLWMLILGFI IAFVLAFSVG ANDVANSFGT AVGSGVVTLK 
61:	QACILASIFE TVGSVLLGAK VSETIRKGLI DVEMYNSTQG LLMAGSVSAM FGSAVWQLVA 
121:	SFLKLPISGT HCIVGATIGF SLVAKGQEGV KWSELIKIVM SWFVSPLLSG IMSGILFFLV 
181:	RAFILHKADP VPNGLRALPV FYACTVGINL FSIMYTGAPL LGFDKLPLWG TILISVGCAV 
241:	FCALIVWFFV CPRMKRKIER EIKCSPSESP LMEKKNSLKE DHEETKLSVG DIENKHPVSE 
301:	VGPATVPLQA VVEERTVSFK LGDLEEAPER ERLPSVDLKE ETSIDSTVNG AVQLPNGNLV 
361:	QFSQAVSNQI NSSGHYQYHT VHKDSGLYKE LLHKLHLAKV GDCMGDSGDK PLRRNNSYTS 
421:	YTMAICGMPL DSFRAKEGEQ KGEEMEKLTW PNADSKKRIR MDSYTSYCNA VSDLHSASEI 
481:	DMSVKAEMGL GDRKGSNGSL EEWYDQDKPE VSLLFQFLQI LTACFGSFAH GGNDVSNAIG 
541:	PLVALYLVYD TGDVSSKVAT PIWLLLYGGV GICVGLWVWG RRVIQTMGKD LTPITPSSGF 
601:	SIELASALTV VIASNIGLPI STTHCKVGSV VSVGWLRSKK AVDWRLFRNI FMAWFVTVPI 
661:	SGVISAAIMA IFRYVILRM