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2.A.21.8.2
High affinity choline transporter 1 (Hemicholinium-3-sensitive choline transporter) (CHT1) (Solute carrier family 5 member 7).  It is required for synthesis of acetyl choline in cholinergic nerve terminals.  It's 13 TMS topology has been verified with an extracellular N-terminus and an intracellular C-terminus.  It is likely to be a homooligomer (Okuda et al. 2012).  It is defective in hereditary motor neuropathy (Barwick et al. 2012).

Accession Number:Q9GZV3
Protein Name:High affinity choline transporter 1
Length:580
Molecular Weight:63204.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:13
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Choline

Cross database links:

Genevestigator: Q9GZV3
eggNOG: COG0591
HEGENOM: HOG000016386
Entrez Gene ID: 60482   
Pfam: PF00474   
KEGG: hsa:60482   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005307 F:choline:sodium symporter activity
GO:0008292 P:acetylcholine biosynthetic process
GO:0007274 P:neuromuscular synaptic transmission
GO:0007269 P:neurotransmitter secretion
GO:0007271 P:synaptic transmission, cholinergic

References (5)

[1] “Molecular cloning of a human, hemicholinium-3-sensitive choline transporter.”  Apparsundaram S.et.al.   11027560
[2] “Functional characterization of the human high-affinity choline transporter.”  Okuda T.et.al.   11068039
[3] “Generation and annotation of the DNA sequences of human chromosomes 2 and 4.”  Hillier L.W.et.al.   15815621
[4] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[5] “Single nucleotide polymorphism of the human high affinity choline transporter alters transport rate.”  Okuda T.et.al.   12237312

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MAFHVEGLIA IIVFYLLILL VGIWAAWRTK NSGSAEERSE AIIVGGRDIG LLVGGFTMTA 
61:	TWVGGGYING TAEAVYVPGY GLAWAQAPIG YSLSLILGGL FFAKPMRSKG YVTMLDPFQQ 
121:	IYGKRMGGLL FIPALMGEMF WAAAIFSALG ATISVIIDVD MHISVIISAL IATLYTLVGG 
181:	LYSVAYTDVV QLFCIFVGLW ISVPFALSHP AVADIGFTAV HAKYQKPWLG TVDSSEVYSW 
241:	LDSFLLLMLG GIPWQAYFQR VLSSSSATYA QVLSFLAAFG CLVMAIPAIL IGAIGASTDW 
301:	NQTAYGLPDP KTTEEADMIL PIVLQYLCPV YISFFGLGAV SAAVMSSADS SILSASSMFA 
361:	RNIYQLSFRQ NASDKEIVWV MRITVFVFGA SATAMALLTK TVYGLWYLSS DLVYIVIFPQ 
421:	LLCVLFVKGT NTYGAVAGYV SGLFLRITGG EPYLYLQPLI FYPGYYPDDN GIYNQKFPFK 
481:	TLAMVTSFLT NICISYLAKY LFESGTLPPK LDVFDAVVAR HSEENMDKTI LVKNENIKLD 
541:	ELALVKPRQS MTLSSTFTNK EAFLDVDSSP EGSGTEDNLQ