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2.A.22.1.7
Dopamine transporter.  The 3-d structure is known to 3.0 Å resolution (Penmatsa et al. 2013).  The crystal structure, bound to the tricyclic antidepressant nortriptyline, shows the transporter locked in an outward-open conformation with nortriptyline wedged between transmembrane helices 1, 3, 6 and 8, blocking the transporter from binding substrate and from isomerizing to an inward-facing conformation. Although the overall structure is similar to that of its prokaryotic relative LeuT, there are multiple distinctions, including a kink in transmembrane helix 12 halfway across the membrane bilayer, a latch-like carboxy-terminal helix that caps the cytoplasmic gate, and a cholesterol molecule wedged within a groove formed by transmembrane helices 1a, 5 and 7.

Accession Number:Q7K4Y6
Protein Name:Transporter
Length:631
Molecular Weight:70330.00
Species:Drosophila melanogaster (Fruit fly) [7227]
Number of TMSs:11
Substrate dopamine

Cross database links:

Structure:
4M48   4XP4   4XP9   4XPB   4XPT     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSPTGHISKS KTPTPHDNDN NSISDERETW SGKVDFLLSV IGFAVDLANV WRFPYLCYKN 
61:	GGGAFLVPYG IMLVVGGIPL FYMELALGQH NRKGAITCWG RLVPLFKGIG YAVVLIAFYV 
121:	DFYYNVIIAW SLRFFFASFT NSLPWTSCNN IWNTPNCRPF ESQNASRVPV IGNYSDLYAM 
181:	GNQSLLYNET YMNGSSLDTS AVGHVEGFQS AASEYFNRYI LELNRSEGIH DLGAIKWDMA 
241:	LCLLIVYLIC YFSLWKGIST SGKVVWFTAL FPYAVLLILL IRGLTLPGSF LGIQYYLTPN 
301:	FSAIYKAEVW VDAATQVFFS LGPGFGVLLA YASYNKYHNN VYKDALLTSF INSATSFIAG 
361:	FVIFSVLGYM AHTLGVRIED VATEGPGLVF VVYPAAIATM PASTFWALIF FMMLLTLGLD 
421:	SSFGGSEAII TALSDEFPKI KRNRELFVAG LFSLYFVVGL ASCTQGGFYF FHLLDRYAAG 
481:	YSILVAVFFE AIAVSWIYGT NRFSEDIRDM IGFPPGRYWQ VCWRFVAPIF LLFITVYGLI 
541:	GYEPLTYADY VYPSWANALG WCIAGSSVVM IPAVAIFKLL STPGSLRQRF TILTTPWRDQ 
601:	QSMAMVLNGV TTEVTVVRLT DTETAKEPVD V