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2.A.22.3.2
γ-aminobutyric acid (GABA):Na+:Cl- symporter, GAT-1 (Stoichiometry, GABA:Na+ = 1:2 where both Na+ binding sites, Na1 and Na2, have been identified. Na2 but not Na1 can accommodate Li+ (Zhou et al., 2006)). Glutamine 291 is essential for Cl- binding (Ben-Yona et al., 2011). Four human isoforms have been identified, GAT-1, GAT-2, GAT-3, and GAT-4, all about 70% identical to each other (Borden et al., 1992). GAT-2 transports γ-aminobutyric acid and β-alanine (Christiansen et al, 2007) It also concentratively takes up β-alanine and α-fluoro-β-alanine (Liu et al., 1999). GAT1 is capable of intracellular Na+-, Cl-- and GABA-induced outward currents (reverse GABA transport; GABA efflux) (Bertram et al., 2011). An acidic amino acid residue in transmembrane helix 10 conserved in the Neurotransmitter:Sodium:Symporters is essential for the formation of the extracellular gate of GAT-1 (Ben-Yona and Kanner, 2012). It is required for stringent gating and tight coupling of ion- and substrate-fluxes in the GABA transporter family (Dayan et al. 2017). GAT-1 is the target of the antiepileptic drug, tiagabine (Kardos et al. 2010). The monomeric protein has been purified fused to GFP (Hu et al. 2017).

Accession Number:P30531
Protein Name:NTG1 aka GAT-1 aka SLC6A1 aka GABATR aka GABT1 aka GAT1
Length:599
Molecular Weight:67074.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate gamma-aminobutyric acid (GABA)

Cross database links:

Genevestigator: P30531 P30531
eggNOG: prNOG16425 COG0733
HEGENOM: HBG702834 HOG000116406
RefSeq: NP_003033.3   
Entrez Gene ID: 6529   
Pfam: PF00209   
OMIM: 137165  gene
KEGG: hsa:6529    hsa:6529   

Gene Ontology

GO:0005887 C:integral to plasma membrane
GO:0005332 F:gamma-aminobutyric acid:sodium symporter ac...
GO:0005328 F:neurotransmitter:sodium symporter activity
GO:0006836 P:neurotransmitter transport
GO:0007268 P:synaptic transmission
GO:0030424 C:axon
GO:0009986 C:cell surface
GO:0016021 C:integral to membrane
GO:0005624 C:membrane fraction
GO:0005886 C:plasma membrane
GO:0019717 C:synaptosome
GO:0005332 F:gamma-aminobutyric acid:sodium symporter activity
GO:0051939 P:gamma-aminobutyric acid import
GO:0007612 P:learning
GO:0032229 P:negative regulation of synaptic transmission, GABAergic
GO:0007269 P:neurotransmitter secretion
GO:0014054 P:positive regulation of gamma-aminobutyric acid secretion
GO:0051260 P:protein homooligomerization
GO:0051592 P:response to calcium ion
GO:0042220 P:response to cocaine
GO:0032355 P:response to estradiol stimulus
GO:0010288 P:response to lead ion
GO:0010243 P:response to organic nitrogen
GO:0014074 P:response to purine-containing compound
GO:0009744 P:response to sucrose stimulus
GO:0009636 P:response to toxin
GO:0055085 P:transmembrane transport

References (6)

[1] “Cloning of the human brain GABA transporter.”  Nelson H.et.al.   2387399
[2] “The DNA sequence, annotation and analysis of human chromosome 3.”  Muzny D.M.et.al.   16641997
[3] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[4] “Cloning of the human brain GABA transporter.”  Nelson H.et.al.   2387399
[5] “The DNA sequence, annotation and analysis of human chromosome 3.”  Muzny D.M.et.al.   16641997
[6] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334

External Searches:

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MATNGSKVAD GQISTEVSEA PVANDKPKTL VVKVQKKAAD LPDRDTWKGR FDFLMSCVGY 
61:	AIGLGNVWRF PYLCGKNGGG AFLIPYFLTL IFAGVPLFLL ECSLGQYTSI GGLGVWKLAP 
121:	MFKGVGLAAA VLSFWLNIYY IVIISWAIYY LYNSFTTTLP WKQCDNPWNT DRCFSNYSMV 
181:	NTTNMTSAVV EFWERNMHQM TDGLDKPGQI RWPLAITLAI AWILVYFCIW KGVGWTGKVV 
241:	YFSATYPYIM LIILFFRGVT LPGAKEGILF YITPNFRKLS DSEVWLDAAT QIFFSYGLGL 
301:	GSLIALGSYN SFHNNVYRDS IIVCCINSCT SMFAGFVIFS IVGFMAHVTK RSIADVAASG 
361:	PGLAFLAYPE AVTQLPISPL WAILFFSMLL MLGIDSQFCT VEGFITALVD EYPRLLRNRR 
421:	ELFIAAVCII SYLIGLSNIT QGGIYVFKLF DYYSASGMSL LFLVFFECVS ISWFYGVNRF 
481:	YDNIQEMVGS RPCIWWKLCW SFFTPIIVAG VFIFSAVQMT PLTMGNYVFP KWGQGVGWLM 
541:	ALSSMVLIPG YMAYMFLTLK GSLKQRIQVM VQPSEDIVRP ENGPEQPQAG SSTSKEAYI