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2.A.22.6.1
Na+/Amino acid transporter 1, SIT1/IMINO (SLC6A20). Transports imino acids such as proline (Km=0.2 mM), pipecolate, and N-methylated amino acids such as MeAIB and sarcosine (Na+-dependent, Cl--stimulated, pH-independent, voltage-dependent) (Li+, but not H+ can substitute for Na+) (Takanaga et al., 2005). It is a 2Na+/1Cl--proline cotransporter (Bröer et al., 2009).

Accession Number:Q64093
Protein Name:Sodium- and chloride-dependent transporter XTRP3 (Solute carrier family 6 member 20) (Neurotransmitter transporter rB21A)
Length:616
Molecular Weight:68971.00
Species:Rattus norvegicus (Rat) [10116]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Apical cell membrane1 / Multi-pass membrane protein2
Substrate Amino acids, Na+, Cl-, Li+, Pipecolate, MeAIB, Sarcosine, Proline

Cross database links:

Genevestigator: Q64093
eggNOG: roNOG09907
RefSeq: NP_579830.1   
Entrez Gene ID: 113918   
Pfam: PF00209   
KEGG: rno:113918   

Gene Ontology

GO:0016324 C:apical plasma membrane
GO:0005887 C:integral to plasma membrane
GO:0015171 F:amino acid transmembrane transporter activity
GO:0005328 F:neurotransmitter:sodium symporter activity
GO:0006865 P:amino acid transport
GO:0006836 P:neurotransmitter transport

References (2)

[1] “Molecular cloning of an orphan transporter. A new member of the neurotransmitter transporter family.”  Smith K.E.et.al.   8001687
[2] “Identification of mammalian proline transporter SIT1 (SLC6A20) with characteristics of classical system imino.”  Takanaga H.et.al.   15632147

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MRLAIKRRAS RGQRPGPDEK RARDMEKARP QWGNPLQFVF ACISYAVGLG NVWRFPYLCQ 
61:	MYGGGSFLVP YLIMLIVEGM PLLYLELAVG QRMRQGSIGA WRTISPYLSG VGVASVVVSF 
121:	FLSMYYNVIN AWGFWYLFHS FQDPLPWSVC PLNSNRTGYD EECEKASSTQ YFWYRKTLNI 
181:	SPSIQENGGV QWEPALCLTL AWLMVYLCIL RGTESTGKVV YFTALMPYCV LIIYLVRGLT 
241:	LHGATNGLMY MFTPKIEQLA NPKAWINAAT QIFFSLGLGF GSLIAFASYN EPSNDCQKHA 
301:	VIVSVINSST SIFASIVTFS IYGFKATFNY ENCLNKVILL LTNSFDLEDG FLTASNLEEV 
361:	KDYLASTYPN KYSEVFPHIR NCSLESELNT AVQGTGLAFI VYAEAIKNME VSQLWSVLYF 
421:	FMLLMLGMGS MLGNTAAILT PLTDSKVISS YLPKEAISGL VCLINCAVGM VFTMEAGNYW 
481:	FDIFNDYAAT LSLLLIVLVE TIAVCYVYGL RRFESDLRAM TGRPLNWYWK AMWAFVSPLL 
541:	IIGLFIFYLS DYILTGTLQY QAWDATQGQL VTKDYPPHAL AVIGLLVASS TMCIPLVALG 
601:	TFIRNRLKRG GSSPVA