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2.A.23.2.2
Glutamate/aspartate:Na+ symporter, GLT1; GLUT-R; EAAT2. Interacts directly with the Na+, K+-ATPase (TC #3.A.3.1) (Rose et al., 2009). Cotransports glutamic acid with three Na+ followed by countertransport of K+ (Teichman et al., 2009). The C-terminal 74aa domain regulates transport activity (Leinenweber et al., 2011). Hippocampal glutamate transporter 1 (GLT-1) levels parallel memory training (Heo et al., 2011). GLT-1 is regulated by MAGI-1 (Zou et al., 2011).  Venom from the spider Parawixia bistriata and a purified compound (Parawixin1) stimulate EAAT2 activity and protect retinal tissue from ischemic damage (Mortensen et al. 2015).  Determinants of this stimulation are at the interface of the trimerization and substrate transport domains ((Mortensen et al. 2015). TMS4 of GLT-1 undergoes a complex conformational shift during substrate translocation (Rong et al. 2016).  

Accession Number:P31596
Protein Name:EAA2 aka SLC1A2 aka EAAT2 aka GLT1
Length:573
Molecular Weight:62106.00
Species:Rattus norvegicus (Rat) [10116]
Number of TMSs:9
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate glutamate, aspartate, Na+, K+

Cross database links:

Genevestigator: P31596
eggNOG: roNOG10707
RefSeq: NP_058911.2   
Entrez Gene ID: 29482   
Pfam: PF00375   

Gene Ontology

GO:0043198 C:dendritic shaft
GO:0043197 C:dendritic spine
GO:0016021 C:integral to membrane
GO:0005624 C:membrane fraction
GO:0042734 C:presynaptic membrane
GO:0005314 F:high-affinity glutamate transmembrane trans...
GO:0017153 F:sodium:dicarboxylate symporter activity
GO:0006835 P:dicarboxylic acid transport
GO:0006836 P:neurotransmitter transport

References (5)

[1] “Cloning and expression of a rat brain L-glutamate transporter.”  Pines G.et.al.   1448170
[2] “Glutamate transporters from brain. A novel neurotransmitter transporter family.”  Kanner B.I.et.al.   8099882
[3] “The rat hepatoma cell line H4-II-E-C3 expresses high activities of the high-affinity glutamate transporter GLT-1A.”  Pollard M.et.al.   11068035
[4] “Histidine 326 is critical for the function of GLT-1, a (Na+ + K+)-coupled glutamate transporter from rat brain.”  Zhang Y.et.al.   7913472
[5] “The amino terminus of the glial glutamate transporter GLT-1 interacts with the LIM protein Ajuba.”  Marie H.et.al.   11860269

External Searches:

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MASTEGANNM PKQVEVRMHD SHLSSEEPKH RNLGMRMCDK LGKNLLLSLT VFGVILGAVC 
61:	GGLLRLAAPI HPDVVMLIAF PGDILMRMLK MLILPLIISS LITGLSGLDA KASGRLGTRA 
121:	MVYYMSTTII AAVLGVILVL AIHPGNPKLK KQLGPGKKND EVSSLDAFLD LIRNLFPENL 
181:	VQACFQQIQT VTKKVLVAPP SEEANTTKAV ISLLNETMNE APEETKIVIK KGLEFKDGMN 
241:	VLGLIGFFIA FGIAMGKMGE QAKLMVEFFN ILNEIVMKLV IMIMWYSPLG IACLICGKII 
301:	AIKDLEVVAR QLGMYMITVI VGLIIHGGIF LPLIYFVVTR KNPFSFFAGI FQAWITALGT 
361:	ASSAGTLPVT FRCLEDNLGI DKRVTRFVLP VGATINMDGT ALYEAVAAIF IAQMNGVILD 
421:	GGQIVTVSLT ATLASIGAAS IPSAGLVTML LILTAVGLPT EDISLLVAVD WLLDRMRTSV 
481:	NVVGDSFGAG IVYHLSKSEL DTIDSQHRMH EDIEMTKTQS VYDDTKNHRE SNSNQCVYAA 
541:	HNSVVIDECK VTLAANGKSA DCSVEEEPWK REK