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2.A.23.2.6
Excitatory amino acid transporter 1 (EAAT1) (Sodium-dependent glutamate/aspartate transporter 1) (GLAST-1) (Solute carrier family 1 member 3).  Mutations cause episodic ataxia type 6 (EA6) (Choi et al. 2016).  EAAT1 regulates the extent and duration of glutamate-mediated signals by the clearance of glutamate after synaptic release. It also has an anion channel activity that prevents additional glutamate release.

Accession Number:P43003
Protein Name:Excitatory amino acid transporter 1
Length:542
Molecular Weight:59572.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:10
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate glutamate, aspartate, Na+

Cross database links:

Genevestigator: P43003
eggNOG: COG1301
HEGENOM: HOG000208776
Entrez Gene ID: 6507   
Pfam: PF00375   
Drugbank: Drugbank Link   
KEGG: hsa:6507   

Gene Ontology

GO:0016323 C:basolateral plasma membrane
GO:0009986 C:cell surface
GO:0043197 C:dendritic spine
GO:0043205 C:fibril
GO:0016021 C:integral to membrane
GO:0005624 C:membrane fraction
GO:0005743 C:mitochondrial inner membrane
GO:0043025 C:neuronal cell body
GO:0005886 C:plasma membrane
GO:0045202 C:synapse
GO:0016595 F:glutamate binding
GO:0005314 F:high-affinity glutamate transmembrane transporter activity
GO:0005313 F:L-glutamate transmembrane transporter activity
GO:0017153 F:sodium:dicarboxylate symporter activity
GO:0031223 P:auditory behavior
GO:0048667 P:cell morphogenesis involved in neuron differentiation
GO:0021545 P:cranial nerve development
GO:0070779 P:D-aspartate import
GO:0009449 P:gamma-aminobutyric acid biosynthetic process
GO:0006537 P:glutamate biosynthetic process
GO:0051938 P:L-glutamate import
GO:0043490 P:malate-aspartate shuttle
GO:0050885 P:neuromuscular process controlling balance
GO:0001504 P:neurotransmitter uptake
GO:0050806 P:positive regulation of synaptic transmission
GO:0046677 P:response to antibiotic
GO:0042493 P:response to drug
GO:0010035 P:response to inorganic substance
GO:0009416 P:response to light stimulus
GO:0009611 P:response to wounding
GO:0007605 P:sensory perception of sound

References (9)

[1] “Cloning and characterization of a glutamate transporter cDNA from human cerebellum.”  Shashidharan P.et.al.   8218410
[2] “Functional comparisons of three glutamate transporter subtypes cloned from human motor cortex.”  Arriza J.L.et.al.   7521911
[3] “Cloning and expression of a human glutamate transporter.”  Kawakami H.et.al.   8123008
[4] “Human high affinity, Na(+)-dependent L-glutamate/L-aspartate transporter GLAST-1 (EAAT-1): gene structure and localization to chromosome 5p11-p12.”  Stoffel W.et.al.   8647279
[5] “A novel alternative splicing form of excitatory amino acid transporter 1 is a negative regulator of glutamate uptake.”  Vallejo-Illarramendi A.et.al.   16042756
[6] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[7] “The DNA sequence and comparative analysis of human chromosome 5.”  Schmutz J.et.al.   15372022
[8] “A quantitative atlas of mitotic phosphorylation.”  Dephoure N.et.al.   18669648
[9] “Mutation in the glutamate transporter EAAT1 causes episodic ataxia, hemiplegia, and seizures.”  Jen J.C.et.al.   16116111

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MTKSNGEEPK MGGRMERFQQ GVRKRTLLAK KKVQNITKED VKSYLFRNAF VLLTVTAVIV 
61:	GTILGFTLRP YRMSYREVKY FSFPGELLMR MLQMLVLPLI ISSLVTGMAA LDSKASGKMG 
121:	MRAVVYYMTT TIIAVVIGII IVIIIHPGKG TKENMHREGK IVRVTAADAF LDLIRNMFPP 
181:	NLVEACFKQF KTNYEKRSFK VPIQANETLV GAVINNVSEA METLTRITEE LVPVPGSVNG 
241:	VNALGLVVFS MCFGFVIGNM KEQGQALREF FDSLNEAIMR LVAVIMWYAP VGILFLIAGK 
301:	IVEMEDMGVI GGQLAMYTVT VIVGLLIHAV IVLPLLYFLV TRKNPWVFIG GLLQALITAL 
361:	GTSSSSATLP ITFKCLEENN GVDKRVTRFV LPVGATINMD GTALYEALAA IFIAQVNNFE 
421:	LNFGQIITIS ITATAASIGA AGIPQAGLVT MVIVLTSVGL PTDDITLIIA VDWFLDRLRT 
481:	TTNVLGDSLG AGIVEHLSRH ELKNRDVEMG NSVIEENEMK KPYQLIAQDN ETEKPIDSET 
541:	KM