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2.A.23.3.2
Insulin-activated amino acid (serine, alanine, glutamate and others):Na+ symporter. Also transports homocysteine (Jiang et al., 2007).  V-9302 is a selective and potent competitive small molecule antagonist of glutamine uptake via ASCT2. Blockage of ASCT2 activity with V-9302 resulted in attenuated cancer cell growth and proliferation, increased cell death, and increased oxidative stress, which collectively contributed to antitumor responses in vitro and in vivo (Schulte et al. 2018).

Accession Number:P51912
Protein Name:AAAX aka SLC1A2 aka SLC1A7 aka ASCT2 aka AAAT
Length:553
Molecular Weight:58483.00
Species:Mus musculus (Mouse) [10090]
Number of TMSs:9
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Serine, Alanine, glutamate, Na+, Homocysteine

Cross database links:

Genevestigator: P51912
eggNOG: maNOG13505
Pfam: PF00375   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0042470 C:melanosome
GO:0015175 F:neutral amino acid transmembrane transporte...
GO:0017153 F:sodium:dicarboxylate symporter activity
GO:0006835 P:dicarboxylic acid transport

References (2)

[1] “Expression of a novel insulin-activated amino acid transporter gene during differentiation of 3T3-L1 preadipocytes into adipocytes.”  Liao K.et.al.   7702599
[2] “Cloning and functional characterization of a system ASC-like Na+-dependent neutral amino acid transporter.”  Utsunomiya-Tate N.et.al.   8662767

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MAVDPPKADP KGVAVDSSRR CPALGSREDQ SAKAGGCCGS RDRVRRCIRA NLLVLLTVAA 
61:	VVAGVGLGLG VSAAGGADAL GPARLTRFAF PGELLLRLLK MIILPLVVCS LIGGAASLDP 
121:	SALGRVGAWA LLFFLVTTLL ASALGVGLAL ALKPGAAVTA ITSINDSVVD PCARSAPTKE 
181:	ALDSFLDLVR NIFPSNLVSA AFRSFATSYE PKDNSCKIPQ SCIQREINST MVQLLCEVEG 
241:	MNILGLVVFA IVFGVALRKL GPEGELLIRF FNSFNDATMV LVSWIMWYAP VGILFLVASK 
301:	IVEMKDVRQL FISLGKYILC CLLGHAIHGL LVLPLIYFLF TRKNPYRFLW GIMTPLATAF 
361:	GTSSSSATLP LMMKCVEEKN GVAKHISRFI LPIGATVNMD GAALFQCVAA VFIAQLNGVS 
421:	LDFVKIITIL VTATASSVGA AGIPAGGVLT LAIILEAVSL PVKDISLILA VDWLVDRSCT 
481:	VLNVEGDAFG AGLLQSYVDR TKMPSSEPEL IQVKNEVSLN PLPLATEEGN PLLKQYQGPT 
541:	GDSSATFEKE SVM