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2.A.23.3.3
Broad-specificity amino acid:Na+ symporter, LAT1 or SLC7A5 (transports most zwitterionic and dibasic amino acids) (Required for intracellular multiplication of Legionella pneumophila (Wieland et al., 2005). SLC7A5 with accessory protein SLC3A2 (the heavy chain; TC# 8.A.9.2.2) mediates bidirectional transport of amino acids and regulates mTOR and autophagy (Nicklin et al., 2009; Estrach et al. 2014).  LAT1 is the sole transport competent subunit of the heterodimer (Napolitano et al. 2015). l-Leucine inhibits uptake of LAT1 substrates as well as cell growth, and it potentiates the efficacy of bestatin and cisplatin, even at low concentrations (25 muM) (Huttunen et al. 2016).  Transports certain thyroid hormones and their derivativeEstrach et al. 2014).  LAT1 is the sole transport competent subunit of the heterodimer (Napolitano et al. 2015). l-Leucine inhibits uptake of LAT1 substrates as well as cell growth, and it potentiates the efficacy of bestatin and cisplatin, even at low concentrations (25 muM) (Huttunen et al. 2016).  Transports certain thyroid hormones and their derivatives (Krause and Hinz 2017).

Accession Number:Q15758
Protein Name:AAAT aka System B° aka ATB° aka NBB aka SLC1A5 aka ASCT2 aka RDRC aka RDR aka M7V1
Length:541
Molecular Weight:56598.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:10
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Amino acids, Thyroid hormones, thyroid hormone derivatives

Cross database links:

Genevestigator: Q15758 Q15758
eggNOG: prNOG11378 COG1301
RefSeq: NP_005619.1   
Entrez Gene ID: 6510   
Pfam: PF00375   
Drugbank: Drugbank Link   
OMIM: 109190  gene
KEGG: hsa:6510   

Gene Ontology

GO:0005887 C:integral to plasma membrane
GO:0042470 C:melanosome
GO:0005624 C:membrane fraction
GO:0015175 F:neutral amino acid transmembrane transporte...
GO:0005515 F:protein binding
GO:0004872 F:receptor activity
GO:0017153 F:sodium:dicarboxylate symporter activity
GO:0006835 P:dicarboxylic acid transport
GO:0015804 P:neutral amino acid transport
GO:0005794 C:Golgi apparatus
GO:0015186 F:L-glutamine transmembrane transporter activity
GO:0006811 P:ion transport

References (25)

[1] “Cloning of the sodium-dependent, broad-scope, neutral amino acid transporter Bo from a human placental choriocarcinoma cell line.”  Kekuda R.et.al.   8702519
[2] “The RD114/simian type D retrovirus receptor is a neutral amino acid transporter.”  Rasko J.E.J.et.al.   10051606
[3] “A sodium-dependent neutral-amino-acid transporter mediates infections of feline and baboon endogenous retroviruses and simian type D retroviruses.”  Tailor C.S.et.al.   10196349
[4] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[5] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[6] “Truncated forms of the dual function human ASCT2 neutral amino acid transporter/retroviral receptor are translationally initiated at multiple alternative CUG and GUG codons.”  Tailor C.S.et.al.   11350958
[7] “Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.”  Olsen J.V.et.al.   17081983
[8] “Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes.”  Chi A.et.al.   17081065
[9] “A quantitative atlas of mitotic phosphorylation.”  Dephoure N.et.al.   18669648
[10] “Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.”  Gauci S.et.al.   19413330
[11] “Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins.”  Wollscheid B.et.al.   19349973
[12] “Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.”  Mayya V.et.al.   19690332
[13] “Cloning of the sodium-dependent, broad-scope, neutral amino acid transporter Bo from a human placental choriocarcinoma cell line.”  Kekuda R.et.al.   8702519
[14] “The RD114/simian type D retrovirus receptor is a neutral amino acid transporter.”  Rasko J.E.J.et.al.   10051606
[15] “A sodium-dependent neutral-amino-acid transporter mediates infections of feline and baboon endogenous retroviruses and simian type D retroviruses.”  Tailor C.S.et.al.   10196349
[16] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[17] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[18] “Truncated forms of the dual function human ASCT2 neutral amino acid transporter/retroviral receptor are translationally initiated at multiple alternative CUG and GUG codons.”  Tailor C.S.et.al.   11350958
[19] “Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.”  Olsen J.V.et.al.   17081983
[20] “Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes.”  Chi A.et.al.   17081065
[21] “A quantitative atlas of mitotic phosphorylation.”  Dephoure N.et.al.   18669648
[22] “Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.”  Gauci S.et.al.   19413330
[23] “Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins.”  Wollscheid B.et.al.   19349973
[24] “Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.”  Mayya V.et.al.   19690332
[25] “Initial characterization of the human central proteome.”  Burkard T.R.et.al.   21269460

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MVADPPRDSK GLAAAEPTAN GGLALASIED QGAAAGGYCG SRDQVRRCLR ANLLVLLTVV 
61:	AVVAGVALGL GVSGAGGALA LGPERLSAFV FPGELLLRLL RMIILPLVVC SLIGGAASLD 
121:	PGALGRLGAW ALLFFLVTTL LASALGVGLA LALQPGAASA AINASVGAAG SAENAPSKEV 
181:	LDSFLDLARN IFPSNLVSAA FRSYSTTYEE RNITGTRVKV PVGQEVEGMN ILGLVVFAIV 
241:	FGVALRKLGP EGELLIRFFN SFNEATMVLV SWIMWYAPVG IMFLVAGKIV EMEDVGLLFA 
301:	RLGKYILCCL LGHAIHGLLV LPLIYFLFTR KNPYRFLWGI VTPLATAFGT SSSSATLPLM 
361:	MKCVEENNGV AKHISRFILP IGATVNMDGA ALFQCVAAVF IAQLSQQSLD FVKIITILVT 
421:	ATASSVGAAG IPAGGVLTLA IILEAVNLPV DHISLILAVD WLVDRSCTVL NVEGDALGAG 
481:	LLQNYVDRTE SRSTEPELIQ VKSELPLDPL PVPTEEGNPL LKHYRGPAGD ATVASEKESV 
541:	M