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2.A.23.4.1
Serine/threonine:Na+ symporter, SstT

Accession Number:P0AGE4
Protein Name:Inner membrane symporter SstT aka YgjU aka B3089
Length:414
Molecular Weight:43478.00
Species:Escherichia coli [83333]
Number of TMSs:9
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Serine, Threonine, Na+

Cross database links:

Genevestigator: P0AGE4
EchoBASE: EB2590
EcoGene: EG12732
eggNOG: COG3633
HEGENOM: HBG289387
RefSeq: AP_003638.1    NP_417560.1   
Entrez Gene ID: 947605   
Pfam: PF00375   
BioCyc: EcoCyc:YGJU-MONOMER    ECOL168927:B3089-MONOMER   
KEGG: ecj:JW3060    eco:b3089   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005295 F:neutral amino acid:sodium symporter activity
GO:0017153 F:sodium:dicarboxylate symporter activity
GO:0006835 P:dicarboxylic acid transport
GO:0032329 P:serine transport

References (5)

[1] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[2] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[3] “Purification, reconstitution, and characterization of Na(+)/serine symporter, SstT, of Escherichia coli.”  Kim Y.-M.et.al.   12097162
[4] “Cloning and expression of the gene for the Na+-coupled serine transporter from Escherichia coli and characteristics of the transporter.”  Ogawa W.et.al.   9852024
[5] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MTTQRSPGLF RRLAHGSLVK QILVGLVLGI LLAWISKPAA EAVGLLGTLF VGALKAVAPI 
61:	LVLMLVMASI ANHQHGQKTN IRPILFLYLL GTFSAALAAV VFSFAFPSTL HLSSSAGDIS 
121:	PPSGIVEVMR GLVMSMVSNP IDALLKGNYI GILVWAIGLG FALRHGNETT KNLVNDMSNA 
181:	VTFMVKLVIR FAPIGIFGLV SSTLATTGFS TLWGYAQLLV VLVGCMLLVA LVVNPLLVWW 
241:	KIRRNPFPLV LLCLRESGVY AFFTRSSAAN IPVNMALCEK LNLDRDTYSV SIPLGATINM 
301:	AGAAITITVL TLAAVNTLGI PVDLPTALLL SVVASLCACG ASGVAGGSLL LIPLACNMFG 
361:	ISNDIAMQVV AVGFIIGVLQ DSCETALNSS TDVLFTAAAC QAEDDRLANS ALRN