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2.A.24.2.3
Malate:Na+ symporter

Accession Number:O05256
Protein Name:YufR aka MAEN
Length:448
Molecular Weight:47824.00
Species:Bacillus subtilis [1423]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate malate, Na+

Cross database links:

HEGENOM: HBG304840
RefSeq: NP_391036.1   
Entrez Gene ID: 938855   
Pfam: PF03390   
KEGG: bsu:BSU31580   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0008514 F:organic anion transmembrane transporter act...
GO:0015293 F:symporter activity
GO:0015711 P:organic anion transport
GO:0006814 P:sodium ion transport

References (4)

[1] “Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai).”  Oudega B.et.al.   9274030
[2] “The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.”  Kunst F.et.al.   9384377
[3] “Bacillus subtilis YqkI is a novel malic/Na+-lactate antiporter that enhances growth on malate at low protonmotive force.”  Wei Y.et.al.   10903309
[4] “The Bacillus subtilis YufLM two-component system regulates the expression of the malate transporters MaeN (YufR) and YflS, and is essential for utilization of malate in minimal medium.”  Tanaka K.et.al.   12949159

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MGAIPKTGTI SPEQKDSQEK NLFQKIWSWE IGVIPLPLYT VLAVIIILAA YYNELPANML 
61:	GGFAIIMILG VFLGDIGQRI PILKDIGGPA ILSLFVPSFL VFYNVLNSTS LDAVTNLMKT 
121:	SNFLYFYIAC LVVGSILGMN RIVLIQGFIR MFVPLVAGTI AAVAAGILVG FIFGYSAYDS 
181:	FFFVVVPIIA GGIGEGILPL SIAYSQILGS SADVFVSQLV PAAIIGNVFA IICAALMKKL 
241:	GDKRPDLNGN GRLVKSKKAN EIFNQKEAEA KIDFKLMGAG VLLACTFFIF GGLLEKFIFI 
301:	PGAILMIISA AAVKYANILP KKMEEGAYQL YKFISSSFTW PLMVGLGILF IPLDDVASVI 
361:	SIPFVIICIS VVIAMIGSGY FVGKLMNMYP VESAIVTCCH SGLGGTGDVA ILSASGRMGL 
421:	MPFAQISTRL GGAGTVICAT VLLRFFTS