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2.A.27.1.1
Glutamate:Na+ symporter (transports L- and D-glutamate, α-methylglutamate and homocysteate). Swapping the order of the two halves (repeat units) does not decrease activity (Dobrowolski and Lolkema, 2010).

Accession Number:P0AER8
Protein Name:Sodium/glutamate symport carrier protein GltS aka GLTC aka B3653
Length:401
Molecular Weight:42425.00
Species:Escherichia coli [83333]
Number of TMSs:10
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate glutamate

Cross database links:

Genevestigator: P0AER8
EchoBASE: EB0401
EcoGene: EG10406
eggNOG: COG0786
HEGENOM: HBG644193
DIP: DIP-48023N
RefSeq: AP_004139.1    NP_418110.1   
Entrez Gene ID: 948166   
Pfam: PF03616   
BioCyc: EcoCyc:GLTS-MONOMER    ECOL168927:B3653-MONOMER   
KEGG: ecj:JW3628    eco:b3653   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015501 F:glutamate:sodium symporter activity
GO:0015813 P:L-glutamate transport
GO:0006814 P:sodium ion transport

References (7)

[1] “Nucleotide sequence of gltS, the Na+/glutamate symport carrier gene of Escherichia coli B.”  Deguchi Y.et.al.   2254324
[2] “Characterization of the Escherichia coli K12 gltS glutamate permease gene.”  Kalman M.et.al.   2017136
[3] “DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication.”  Burland V.D.et.al.   7686882
[4] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[5] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[6] “The nucleotide sequence of recG, the distal spo operon gene in Escherichia coli K-12.”  Kalman M.et.al.   1544582
[7] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MFHLDTLATL VAATLTLLLG RKLVHSVSFL KKYTIPEPVA GGLLVALALL VLKKSMGWEV 
61:	NFDMSLRDPL MLAFFATIGL NANIASLRAG GRVVGIFLIV VVGLLVMQNA IGIGMASLLG 
121:	LDPLMGLLAG SITLSGGHGT GAAWSKLFIE RYGFTNATEV AMACATFGLV LGGLIGGPVA 
181:	RYLVKHSTTP NGIPDDQEVP TAFEKPDVGR MITSLVLIET IALIAICLTV GKIVAQLLAG 
241:	TAFELPTFVC VLFVGVILSN GLSIMGFYRV FERAVSVLGN VSLSLFLAMA LMGLKLWELA 
301:	SLALPMLAIL VVQTIFMALY AIFVTWRMMG KNYDAAVLAA GHCGFGLGAT PTAIANMQAI 
361:	TERFGPSHMA FLVVPMVGAF FIDIVNALVI KLYLMLPIFA G