TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


2.A.28.1.2
Liver/ileal bile acid:Na+ symporter, ASBT or NTCP (SLC10A2) (essential for liver or intestinal bile acid transport and homeostasis (Rao et al., 2008). TMS4 forms part of the substrate translocation pathway (Khantwal and Swaan, 2008); TMS7 plays a role in substrate binding and translocation (González et al., 2012); TMS1 contributes to substrate translocation and protein stability (da Silva et al., 2011), and TMS2 coordinates Na+ translocation (Sabit et al. 2013).  NTCP serves as the Hepatitis B Virus (HBV) receptor, and drugs developed to target NTCP induce autophagy and may provide therapy for HBV (Zhang et al. 2015). Decreased activity leads to luminal bile salt conecentrations and either increased eletrolyte secretion or decresased reabsolption van der Mark et al., 2014. Function and stability depend on N-glycosylation (Muthusamy et al. 2015).  Specific inhibitors are known (Slijepcevic and van de Graaf 2017).

Accession Number:Q12908
Protein Name:Ileal sodium/bile acid cotransporter
Length:348
Molecular Weight:37698.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:9
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate bile acids, bile conjugates

Cross database links:

COG0385
Genevestigator: Q12908 Q12908
HEGENOM: HBG714579 HOG000234524
RefSeq: NP_000443.1   
Entrez Gene ID: 6555   
Pfam: PF01758   
OMIM: 601295  gene+phenotype
KEGG: hsa:6555    hsa:6555   

Gene Ontology

GO:0005887 C:integral to plasma membrane
GO:0008508 F:bile acid:sodium symporter activity
GO:0015711 P:organic anion transport
GO:0006814 P:sodium ion transport
GO:0016324 C:apical plasma membrane
GO:0005634 C:nucleus
GO:0000502 C:proteasome complex
GO:0008206 P:bile acid metabolic process

References (16)

[1] “Identification of a mutation in the ileal sodium-dependent bile acid transporter gene that abolishes transport activity.”  Wong M.H.et.al.   7592981
[2] “Primary bile acid malabsorption caused by mutations in the ileal sodium-dependent bile acid transporter gene (SLC10A2).”  Oelkers P.et.al.   9109432
[3] “Genetic and physiological data implicating the new human gene G72 and the gene for D-amino acid oxidase in schizophrenia.”  Chumakov I.et.al.   12364586
[4] “The DNA sequence and analysis of human chromosome 13.”  Dunham A.et.al.   15057823
[5] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[6] “Topology scanning and putative three-dimensional structure of the extracellular binding domains of the apical sodium-dependent bile acid transporter (SLC10A2).”  Zhang E.Y.et.al.   15350125
[7] “Analysis of the ileal bile acid transporter gene, SLC10A2, in subjects with familial hypertriglyceridemia.”  Love M.W.et.al.   11742882
[8] “Absence of dysfunctional ileal sodium-bile acid cotransporter gene mutations in patients with adult-onset idiopathic bile acid malabsorption.”  Montagnani M.et.al.   11589382
[9] “Identification of a mutation in the ileal sodium-dependent bile acid transporter gene that abolishes transport activity.”  Wong M.H.et.al.   7592981
[10] “Primary bile acid malabsorption caused by mutations in the ileal sodium-dependent bile acid transporter gene (SLC10A2).”  Oelkers P.et.al.   9109432
[11] “Genetic and physiological data implicating the new human gene G72 and the gene for D-amino acid oxidase in schizophrenia.”  Chumakov I.et.al.   12364586
[12] “The DNA sequence and analysis of human chromosome 13.”  Dunham A.et.al.   15057823
[13] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[14] “Topology scanning and putative three-dimensional structure of the extracellular binding domains of the apical sodium-dependent bile acid transporter (SLC10A2).”  Zhang E.Y.et.al.   15350125
[15] “Analysis of the ileal bile acid transporter gene, SLC10A2, in subjects with familial hypertriglyceridemia.”  Love M.W.et.al.   11742882
[16] “Absence of dysfunctional ileal sodium-bile acid cotransporter gene mutations in patients with adult-onset idiopathic bile acid malabsorption.”  Montagnani M.et.al.   11589382

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MNDPNSCVDN ATVCSGASCV VPESNFNNIL SVVLSTVLTI LLALVMFSMG CNVEIKKFLG 
61:	HIKRPWGICV GFLCQFGIMP LTGFILSVAF DILPLQAVVV LIIGCCPGGT ASNILAYWVD 
121:	GDMDLSVSMT TCSTLLALGM MPLCLLIYTK MWVDSGSIVI PYDNIGTSLV ALVVPVSIGM 
181:	FVNHKWPQKA KIILKIGSIA GAILIVLIAV VGGILYQSAW IIAPKLWIIG TIFPVAGYSL 
241:	GFLLARIAGL PWYRCRTVAF ETGMQNTQLC STIVQLSFTP EELNVVFTFP LIYSIFQLAF 
301:	AAIFLGFYVA YKKCHGKNKA EIPESKENGT EPESSFYKAN GGFQPDEK