TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


2.A.3.1.1
Phenylalanine:H+ symporter, PheP of 458 aas and 12 established TMSs (Pi and Pittard 1996; Pi et al. 2002).  Catalytic residues have been identified (Pi et al. 1993), and interhelical interactions have been proposed (Dogovski et al. 2003).

Accession Number:P24207
Protein Name:PheP aka B0576
Length:458
Molecular Weight:50677.00
Species:Escherichia coli [83333]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate phenylalanine

Cross database links:

Genevestigator: P24207
EchoBASE: EB0702
EcoGene: EG10708
eggNOG: COG1113
HEGENOM: HBG492579
DIP: DIP-10479N
RefSeq: AP_001221.1    NP_415108.1   
Entrez Gene ID: 945199   
Pfam: PF00324   
BioCyc: EcoCyc:PHEP-MONOMER    ECOL168927:B0576-MONOMER   
KEGG: ecj:JW0565    eco:b0576   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015171 F:amino acid transmembrane transporter activity
GO:0006865 P:amino acid transport
GO:0055085 P:transmembrane transport

References (6)

[1] “Cloning and sequencing of the pheP gene, which encodes the phenylalanine-specific transport system of Escherichia coli.”  Pi J.et.al.   1711024
[2] “A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.”  Oshima T.et.al.   8905232
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “Topology of the phenylalanine-specific permease of Escherichia coli.”  Pi J.et.al.   8626334
[6] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MKNASTVSED TASNQEPTLH RGLHNRHIQL IALGGAIGTG LFLGIGPAIQ MAGPAVLLGY 
61:	GVAGIIAFLI MRQLGEMVVE EPVSGSFAHF AYKYWGPFAG FLSGWNYWVM FVLVGMAELT 
121:	AAGIYMQYWF PDVPTWIWAA AFFIIINAVN LVNVRLYGET EFWFALIKVL AIIGMIGFGL 
181:	WLLFSGHGGE KASIDNLWRY GGFFATGWNG LILSLAVIMF SFGGLELIGI TAAEARDPEK 
241:	SIPKAVNQVV YRILLFYIGS LVVLLALYPW VEVKSNSSPF VMIFHNLDSN VVASALNFVI 
301:	LVASLSVYNS GVYSNSRMLF GLSVQGNAPK FLTRVSRRGV PINSLMLSGA ITSLVVLINY 
361:	LLPQKAFGLL MALVVATLLL NWIMICLAHL RFRAAMRRQG RETQFKALLY PFGNYLCIAF 
421:	LGMILLLMCT MDDMRLSAIL LPVWIVFLFM AFKTLRRK