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2.A.3.1.13
Putrescine importer, PuuP (Kurihara et al., 2005)

Accession Number:P76037
Protein Name:PuuP
Length:461
Molecular Weight:50853.00
Species:Escherichia coli (strain K12) [83333]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Putrescine

Cross database links:

Genevestigator: P76037
EchoBASE: EB3666
EcoGene: EG13907
eggNOG: COG0531
HEGENOM: HBG634400
DIP: DIP-11601N
RefSeq: AP_001922.1    NP_415812.4   
Entrez Gene ID: 946287   
Pfam: PF00324   
BioCyc: EcoCyc:B1296-MONOMER    ECOL168927:B1296-MONOMER   
KEGG: ecj:JW1289    eco:b1296   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015171 F:amino acid transmembrane transporter activity
GO:0015489 F:putrescine transmembrane transporter activity
GO:0006865 P:amino acid transport
GO:0009447 P:putrescine catabolic process
GO:0015847 P:putrescine transport
GO:0055085 P:transmembrane transport

References (4)

[1] “A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map.”  Aiba H.et.al.   9097039
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “A novel putrescine utilization pathway involves gamma-glutamylated intermediates of Escherichia coli K-12.”  Kurihara S.et.al.   15590624

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MAINSPLNIA AQPGKTRLRK SLKLWQVVMM GLAYLTPMTV FDTFGIVSGI SDGHVPASYL 
61:	LALAGVLFTA ISYGKLVRQF PEAGSAYTYA QKSINPHVGF MVGWSSLLDY LFLPMINVLL 
121:	AKIYLSALFP EVPPWVWVVT FVAILTAANL KSVNLVANFN TLFVLVQISI MVVFIFLVVQ 
181:	GLHKGEGVGT VWSLQPFISE NAHLIPIITG ATIVCFSFLG FDAVTTLSEE TPDAARVIPK 
241:	AIFLTAVYGG VIFIAASFFM QLFFPDISRF KDPDAALPEI ALYVGGKLFQ SIFLCTTFVN 
301:	TLASGLASHA SVSRLLYVMG RDNVFPERVF GYVHPKWRTP ALNVIMVGIV ALSALFFDLV 
361:	TATALINFGA LVAFTFVNLS VFNHFWRRKG MNKSWKDHFH YLLMPLVGAL TVGVLWVNLE 
421:	STSLTLGLVW ASLGGAYLWY LIRRYRKVPL YDGDRTPVSE T