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2.A.3.1.14
Low-affinity putrescine importer PlaP

Accession Number:P0AA47
Protein Name:Low-affinity putrescine importer PlaP
Length:452
Molecular Weight:49538.00
Species:Escherichia coli (strain K12) [83333]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Putrescine

Cross database links:

Genevestigator: P0AA47
EchoBASE: EB1842
EcoGene: EG11896
eggNOG: COG0531
HEGENOM: HOG000164060
Entrez Gene ID: 946533   
Pfam: PF00324   
KEGG: eco:b2014   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015171 F:amino acid transmembrane transporter activity
GO:0015489 F:putrescine transmembrane transporter activity
GO:0015295 F:solute:hydrogen symporter activity
GO:0048870 P:cell motility

References (5)

[1] “A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.”  Itoh T.et.al.   9097040
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996
[5] “A novel putrescine importer required for type 1 pili-driven surface motility induced by extracellular putrescine in Escherichia coli K-12.”  Kurihara S.et.al.   21266585

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSHNVTPNTS RVELRKTLTL VPVVMMGLAY MQPMTLFDTF GIVSGLTDGH VPTAYAFALI 
61:	AILFTALSYG KLVRRYPSAG SAYTYAQKSI SPTVGFMVGW SSLLDYLFAP MINILLAKIY 
121:	FEALVPSIPS WMFVVALVAF MTAFNLRSLK SVANFNTVIV VLQVVLIAVI LGMVVYGVFE 
181:	GEGAGTLAST RPFWSGDAHV IPMITGATIL CFSFTGFDGI SNLSEETKDA ERVIPRAIFL 
241:	TALIGGMIFI FATYFLQLYF PDISRFKDPD ASQPEIMLYV AGKAFQVGAL IFSTITVLAS 
301:	GMAAHAGVAR LMYVMGRDGV FPKSFFGYVH PKWRTPAMNI ILVGAIALLA INFDLVMATA 
361:	LINFGALVAF TFVNLSVISQ FWIREKRNKT LKDHFQYLFL PMCGALTVGA LWVNLEESSM 
421:	VLGLIWAAIG LIYLACVTKS FRNPVPQYED VA