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2.A.3.1.7
D-Serine/D-alanine/glycine/D-cycloserine:H+ symporter (regulated by the small RNA, GcvB; Pulvermacher et al., 2009).  The system is active after growth in minimal medium but not after growth in complex medium (Baisa et al. 2013).

Accession Number:P0AAE0
Protein Name:D-serine/D-alanine/glycine transporter CycA aka DAGA aka B4208 aka JW4166
Length:470
Molecular Weight:51660.00
Species:Escherichia coli [83333]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate D-serine, D-alanine, Glycine, D-cycloserine, H+, Serine

Cross database links:

Genevestigator: P0AAE0
EchoBASE: EB2397
EcoGene: EG12504
eggNOG: COG1113
HEGENOM: HBG492579
RefSeq: AP_004708.1    NP_418629.1   
Entrez Gene ID: 948725   
Pfam: PF00324   
BioCyc: EcoCyc:CYCA-MONOMER    ECOL168927:B4208-MONOMER   
KEGG: ecj:JW4166    eco:b4208   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015171 F:amino acid transmembrane transporter activity
GO:0006865 P:amino acid transport
GO:0055085 P:transmembrane transport

References (4)

[1] “Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes.”  Burland V.D.et.al.   7610040
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MVDQVKVVAD DQAPAEQSLR RNLTNRHIQL IAIGGAIGTG LFMGSGKTIS LAGPSIIFVY 
61:	MIIGFMLFFV MRAMGELLLS NLEYKSFSDF ASDLLGPWAG YFTGWTYWFC WVVTGMADVV 
121:	AITAYAQFWF PDLSDWVASL AVIVLLLTLN LATVKMFGEM EFWFAMIKIV AIVSLIVVGL 
181:	VMVAMHFQSP TGVEASFAHL WNDGGWFPKG LSGFFAGFQI AVFAFVGIEL VGTTAAETKD 
241:	PEKSLPRAIN SIPIRIIMFY VFALIVIMSV TPWSSVVPEK SPFVELFVLV GLPAAASVIN 
301:	FVVLTSAASS ANSGVFSTSR MLFGLAQEGV APKAFAKLSK RAVPAKGLTF SCICLLGGVV 
361:	MLYVNPSVIG AFTMITTVSA ILFMFVWTII LCSYLVYRKQ RPHLHEKSIY KMPLGKLMCW 
421:	VCMAFFVFVV VLLTLEDDTR QALLVTPLWF IALGLGWLFI GKKRAAELRK