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2.A.3.10.12
Leucine sensor/transcription factor. Mutants hyper- and hyposensitive to inducer (Poulsen et al., 2008) suggest a sensor mechanism involving outward and inward facing conformations.

Accession Number:Q03770
Protein Name:Ssy1 aka SHR10 aka YDR160W aka YD8358.14
Length:852
Molecular Weight:95744.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate Leucine

Cross database links:

Genevestigator: Q03770
eggNOG: fuNOG07889
HEGENOM: HBG396224
DIP: DIP-2920N
RefSeq: NP_010444.1   
Entrez Gene ID: 851738   
Pfam: PF00324   
KEGG: sce:YDR160W   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015171 F:amino acid transmembrane transporter activity
GO:0006865 P:amino acid transport
GO:0043200 P:response to amino acid stimulus
GO:0055085 P:transmembrane transport

References (12)

[1] “The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.”  Jacq C.et.al.   9169867
[2] “The permease homologue Ssy1p controls the expression of amino acid and peptide transporter genes in Saccharomyces cerevisiae.”  Didion T.et.al.   9489675
[3] “Mutations in five loci affecting GAP1-independent uptake of neutral amino acids in yeast.”  Joergensen M.U.et.al.   9483800
[4] “Amino acid signaling in Saccharomyces cerevisiae: a permease-like sensor of external amino acids and F-Box protein Grr1p are required for transcriptional induction of the AGP1 gene, which encodes a broad-specificity amino acid permease.”  Iraqui I.et.al.   9891035
[5] “Ssy1p and Ptr3p are plasma membrane components of a yeast system that senses extracellular amino acids.”  Klasson H.et.al.   10409731
[6] “Genetic and biochemical analysis of the yeast plasma membrane Ssy1p-Ptr3p-Ssy5p sensor of extracellular amino acids.”  Forsberg H.et.al.   11154269
[7] “The role of the yeast plasma membrane SPS nutrient sensor in the metabolic response to extracellular amino acids.”  Forsberg H.et.al.   11679080
[8] “Genome-wide expression analysis of genes affected by amino acid sensor Ssy1p in Saccharomyces cerevisiae.”  Kodama Y.et.al.   12073087
[9] “Receptor-mediated endoproteolytic activation of two transcription factors in yeast.”  Andreasson C.et.al.   12502738
[10] “Constitutive and hyperresponsive signaling by mutant forms of Saccharomyces cerevisiae amino acid sensor Ssy1.”  Gaber R.F.et.al.   14555474
[11] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258
[12] “Activation of the SPS amino acid-sensing pathway in Saccharomyces cerevisiae correlates with the phosphorylation state of a sensor component, Ptr3.”  Liu Z.et.al.   17984223

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSSVNQIYDL FPNKHNIQFT DSHSQEHDTS SSLAKNDTDG TISIPGSIDT GILKSIIEEQ 
61:	GWNDAELYRS SIQNQRFFLT DKYTKKKHLT MEDMLSPEEE QIYQEPIQDF QTYNKRVQRE 
121:	YELRERMEEF FRQNTKNDLH ILNEDSLNQQ YSPLGPADYV LPLDRYSRMK HIASNFFRKK 
181:	LGIPRKLKRR SHYNPNAEGH TKGNSSILSS TTDVIDNASY RNIAIDENVD ITHKEHAIDE 
241:	INEQGASGSE SVVEGGSLLH DIEKVFNRSR ATRKYHIQRK LKVRHIQMLS IGACFSVGLF 
301:	LTSGKAFSIA GPFGTLLGFG LTGSIILATM LSFTELSTLI PVSSGFSGLA SRFVEDAFGF 
361:	ALGWTYWISC MLALPAQVSS STFYLSYYNN VNISKGVTAG FITLFSAFSI VVNLLDVSIM 
421:	GEIVYVAGIS KVIIAILMVF TMIILNAGHG NDIHEGVGFR YWDSSKSVRN LTYGLYRPTF 
481:	DLADAGEGSK KGISGPKGRF LATASVMLIS TFAFSGVEMT FLASGEAINP RKTIPSATKR 
541:	TFSIVLISYV FLIFSVGINI YSGDPRLLSY FPGISEKRYE AIIKGTGMDW RLRTNCRGGI 
601:	DYRQISVGTG YSSPWVVALQ NFGLCTFASA FNAILIFFTA TAGISSLFSC SRTLYAMSVQ 
661:	RKAPPVFEIC SKRGVPYVSV IFSSLFSVIA YIAVDQTAIE NFDVLANVSS ASTSIIWMGL 
721:	NLSFLRFYYA LKQRKDIISR NDSSYPYKSP FQPYLAIYGL VGCSLFVIFM GYPNFIHHFW 
781:	STKAFFSAYG GLMFFFISYT AYKVLGTSKI QRLDQLDMDS GRREMDRTDW TEHSQYLGTY 
841:	RERAKKLVTW LI