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2.A.3.10.15
S-adenosylmethionine uptake permease, SAM3 (also takes up polyamines, glutamate, lysine and the toxic S-adenosylmethionine analogue sinefungin) (Uemura et al., 2007; Zheng et al., 2007; Kashiwagi and Igarashi 2011).

Accession Number:Q08986
Protein Name:SAM3 aka YPL274w
Length:587
Molecular Weight:64354.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate S-adenosylmethionine, Polyamines, glutamate, lysine, Sinefungin

Cross database links:

Genevestigator: Q08986
eggNOG: fuNOG08327
HEGENOM: HBG492579
DIP: DIP-8874N
RefSeq: NP_015049.1   
Entrez Gene ID: 855854   
Pfam: PF00324   
KEGG: sce:YPL274W   

Gene Ontology

GO:0005783 C:endoplasmic reticulum
GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005515 F:protein binding
GO:0015489 F:putrescine transmembrane transporter activity
GO:0000095 F:S-adenosylmethionine transmembrane transpor...
GO:0015606 F:spermidine transmembrane transporter activity
GO:0015847 P:putrescine transport
GO:0015805 P:S-adenosylmethionine transport
GO:0015848 P:spermidine transport
GO:0055085 P:transmembrane transport

References (6)

[1] “The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.”  Bussey H.et.al.   9169875
[2] “Transport of sulfonium compounds. Characterization of the s-adenosylmethionine and s-methylmethionine permeases from the yeast Saccharomyces cerevisiae.”  Rouillon A.et.al.   10497160
[3] “Global analysis of protein localization in budding yeast.”  Huh W.-K.et.al.   14562095
[4] “Global analysis of protein expression in yeast.”  Ghaemmaghami S.et.al.   14562106
[5] “Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway.”  Gruhler A.et.al.   15665377
[6] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MDILKRGNES DKFTKIETES TTIPNDSDRS GSLIRRMKDS FKQSNLHVIP EDLENSEQTE 
61:	QEKIQWKLAS QPYQKVLSQR HLTMIAIGGT LGTGLFIGLG YSLASGPAAL LIGFLLVGTS 
121:	MFCVVQSAAE LSCQFPVSGS YATHVSRFID ESVGFTVATN YALAWLISFP SELIGCALTI 
181:	SYWNQTVNPA VWVAIFYVFI MVLNLFGVRG FAETEFALSI IKVIAIFIFI IIGIVLIAGG 
241:	GPNSTGYIGA KYWHDPGAFA KPVFKNLCNT FVSAAFSFGG SELVLLTSTE SKNISAISRA 
301:	AKGTFWRIAI FYITTVVIIG CLVPYNDPRL LSGSNSEDVS ASPFVIALSN TGSMGAKVSN 
361:	FMNVVILVAV VSVCNSCVYA SSRLIQALGA SGQLPSVCSY MDRKGRPLVG IGISGAFGLL 
421:	GFLVASKKED EVFTWLFALC SISSFFTWFC ICMSQIRFRM ALKAQGRSND EIAYKSILGV 
481:	YGGILGCVLN ALLIAGEIYV SAAPVGSPSS AEAFFEYCLS IPIMIVVYFA HRFYRRDWKH 
541:	FYIKRSEIDL DTGCSVENLE LFKAQKEAEE QLIASKPFYY KIYRFWC