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2.A.3.10.19
The high affinity polyamine (spermidine > putrescine)/carnitine, low affinity amino acid transporter, AGP2 (Aouida et al., 2005; Uemura et al., 2007)

Accession Number:P38090
Protein Name:General amino acid permease AGP2
Length:596
Molecular Weight:67262.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Polyamines, Amino acids

Cross database links:

Genevestigator: P38090
eggNOG: fuNOG06480
HEGENOM: HBG492579
DIP: DIP-8141N
RefSeq: NP_009690.1   
Entrez Gene ID: 852429   
Pfam: PF00324   
KEGG: sce:YBR132C   

Gene Ontology

GO:0005789 C:endoplasmic reticulum membrane
GO:0005887 C:integral to plasma membrane
GO:0005774 C:vacuolar membrane
GO:0015171 F:amino acid transmembrane transporter activity
GO:0015226 F:carnitine transporter activity
GO:0015203 F:polyamine transmembrane transporter activity
GO:0005515 F:protein binding
GO:0015879 P:carnitine transport
GO:0006631 P:fatty acid metabolic process
GO:0015846 P:polyamine transport
GO:0006970 P:response to osmotic stress

References (6)

[1] “The sequence of 29.7 kb from the right arm of chromosome II reveals 13 complete open reading frames, of which ten correspond to new genes.”  Becam A.-M.et.al.   8091856
[2] “Complete DNA sequence of yeast chromosome II.”  Feldmann H.et.al.   7813418
[3] “An analysis of the sequence of part of the right arm of chromosome II of S. cerevisiae reveals new genes encoding an amino-acid permease and a carboxypeptidase.”  Nasr F.et.al.   7954890
[4] “Molecular characterization of carnitine-dependent transport of acetyl-CoA from peroxisomes to mitochondria in Saccharomyces cerevisiae and identification of a plasma membrane carnitine transporter, Agp2p.”  van Roermund C.W.et.al.   10545096
[5] “Carnitine uptake by AGP2 in yeast Saccharomyces cerevisiae is dependent on Hog1 MAP kinase pathway.”  Lee J.et.al.   12132580
[6] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MTKERMTIDY ENDGDFEYDK NKYKTITTRI KSIEPSEGWL EPSGSVGHIN TIPEAGDVHV 
61:	DEHEDRGSSI DDDSRTYLLY FTETRRKLEN RHVQLIAISG VIGTALFVAI GKALYRGGPA 
121:	SLLLAFALWC VPILCITVST AEMVCFFPVS SPFLRLATKC VDDSLAVMAS WNFWFLECVQ 
181:	IPFEIVSVNT IIHYWRDDYS AGIPLAVQVV LYLLISICAV KYYGEMEFWL ASFKIILALG 
241:	LFTFTFITML GGNPEHDRYG FRNYGESPFK KYFPDGNDVG KSSGYFQGFL ACLIQASFTI 
301:	AGGEYISMLA GEVKRPRKVL PKAFKQVFVR LTFLFLGSCL CVGIVCSPND PDLTAAINEA 
361:	RPGAGSSPYV IAMNNLKIRI LPDIVNIALI TAAFSAGNAY TYCSSRTFYG MALDGYAPKI 
421:	FTRCNRHGVP IYSVAISLVW ALVSLLQLNS NSAVVLNWLI NLITASQLIN FVVLCIVYLF 
481:	FRRAYHVQQD SLPKLPFRSW GQPYTAIIGL VSCSAMILIQ GYTVFFPKLW NTQDFLFSYL 
541:	MVFINIGIYV GYKFIWKRGK DHFKNPHEID FSKELTEIEN HEIESSFEKF QYYSKA