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2.A.3.2.1
Putrescine:ornithine antiporter for putrescine export; putrescine:H+ symporter for uptake (Igarashi and Kashiwagi 1996). Modeling tools have been used to gain information about the structures and functions of CadB and PotE in E. coli (Tomitori et al., 2012).

Accession Number:P0AAF1
Protein Name:Putrescine-ornithine antiporter PotE aka B0692 aka JW0679
Length:439
Molecular Weight:46495.00
Species:Escherichia coli [83333]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Putrescine

Cross database links:

Genevestigator: P0AAF1
EchoBASE: EB0746
EcoGene: EG10753
eggNOG: COG0531
HEGENOM: HBG586921
RefSeq: AP_001330.1    NP_415219.1   
Entrez Gene ID: 945422   
Pfam: PF00324   
BioCyc: EcoCyc:POTE-MONOMER    ECOL168927:B0692-MONOMER   
KEGG: ecj:JW0679    eco:b0692   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015171 F:amino acid transmembrane transporter activity
GO:0015297 F:antiporter activity
GO:0006865 P:amino acid transport
GO:0055085 P:transmembrane transport

References (5)

[1] “Coexistence of the genes for putrescine transport protein and ornithine decarboxylase at 16 min on Escherichia coli chromosome.”  Kashiwagi K.et.al.   1939141
[2] “A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.”  Oshima T.et.al.   8905232
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSQAKSNKMG VVQLTILTMV NMMGSGIIML PTKLAEVGTI SIISWLVTAV GSMALAWAFA 
61:	KCGMFSRKSG GMGGYAEYAF GKSGNFMANY TYGVSLLIAN VAIAISAVGY GTELLGASLS 
121:	PVQIGLATIG VLWICTVANF GGARITGQIS SITVWGVIIP VVGLCIIGWF WFSPTLYVDS 
181:	WNPHHAPFFS AVGSSIAMTL WAFLGLESAC ANTDVVENPE RNVPIAVLGG TLGAAVIYIV 
241:	STNVIAGIVP NMELANSTAP FGLAFAQMFT PEVGKVIMAL MVMSCCGSLL GWQFTIAQVF 
301:	KSSSDEGYFP KIFSRVTKVD APVQGMLTIV IIQSGLALMT ISPSLNSQFN VLVNLAVVTN 
361:	IIPYILSMAA LVIIQKVANV PPSKAKVANF VAFVGAMYSF YALYSSGEEA MLYGSIVTFL 
421:	GWTLYGLVSP RFELKNKHG