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2.A.3.2.5
Homodimeric electrogenic arginine (Km=80μM):agmatine antiporter, AdiC, involved in extreme acid resistance (Fang et al., 2007; Gong et al., 2003; Iyer et al., 2003). A projection structure at 6.5 Å resolution has been published (Casagrande et al., 2008), and the 3.2 Å resolution X-ray structure was determined by Fang et al., 2009 and Gao et al., 2009. Protonation of glutamate 208 induces release of agmatine in the outward-facing conformation (Zomot and Bahar, 2011). The 3.0 Å structure of an Arg-bound form in an open-to-out conformation completed the picture of the major states of the porter during the transport cycle (Kowalczyk et al., 2011). Aromatic residues may regulate access to both the outward- and inward-facing states (Krammer et al. 2016).

Accession Number:P60061
Protein Name:Arginine/agmatine antiporter
Length:445
Molecular Weight:46843.00
Species:Escherichia coli [83333]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Arginine

Cross database links:

Genevestigator: P60061
EchoBASE: EB2355
EcoGene: EG12462
eggNOG: COG0531
HEGENOM: HBG586921
RefSeq: AP_004616.1    NP_418539.1   
Entrez Gene ID: 948628   
Pfam: PF00324   
BioCyc: EcoCyc:YJDE-MONOMER    ECOL168927:B4115-MONOMER   
KEGG: ecj:JW4076    eco:b4115   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015171 F:amino acid transmembrane transporter activity
GO:0015300 F:solute:solute antiporter activity
GO:0006865 P:amino acid transport
GO:0051454 P:intracellular pH elevation
GO:0001101 P:response to acid
GO:0055085 P:transmembrane transport

References (6)

[1] “Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes.”  Burland V.D.et.al.   7610040
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “YjdE (AdiC) is the arginine:agmatine antiporter essential for arginine-dependent acid resistance in Escherichia coli.”  Gong S.et.al.   12867448
[5] “Arginine-agmatine antiporter in extreme acid resistance in Escherichia coli.”  Iyer R.et.al.   14594828
[6] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996
Structure:
3OB6   3l1l   3lrb   5j4i     

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FASTA formatted sequence
1:	MSSDADAHKV GLIPVTLMVS GNIMGSGVFL LPANLASTGG IAIYGWLVTI IGALGLSMVY 
61:	AKMSFLDPSP GGSYAYARRC FGPFLGYQTN VLYWLACWIG NIAMVVIGVG YLSYFFPILK 
121:	DPLVLTITCV VVLWIFVLLN IVGPKMITRV QAVATVLALI PIVGIAVFGW FWFRGETYMA 
181:	AWNVSGLGTF GAIQSTLNVT LWSFIGVESA SVAAGVVKNP KRNVPIATIG GVLIAAVCYV 
241:	LSTTAIMGMI PNAALRVSAS PFGDAARMAL GDTAGAIVSF CAAAGCLGSL GGWTLLAGQT 
301:	AKAAADDGLF PPIFARVNKA GTPVAGLIIV GILMTIFQLS SISPNATKEF GLVSSVSVIF 
361:	TLVPYLYTCA ALLLLGHGHF GKARPAYLAV TTIAFLYCIW AVVGSGAKEV MWSFVTLMVI 
421:	TAMYALNYNR LHKNPYPLDA PISKD