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2.A.31.1.2
Anion exchanger-2 (AE2; 1241 aas); catalyzes solium-independent Cl-:HCO3- exchange; forms a metabolon with the carbonic anhydrase, Car2 (AAH11949) (Gonzalez-Begne et al., 2007) and with another carbonic anhydrase, CAIX (Q16790) which also forms complexes with AE1 and AE3, activating all of these  transporters about 30% (Morgan et al. 2007).  Cys residues play a role in pH sensitivity, but are not essential for activity (Reimold et al. 2013).

Accession Number:P04920
Protein Name:AE2
Length:1241
Molecular Weight:137009.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:14
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate Cl-, bicarbonate

Cross database links:

Genevestigator: P04920 P04920
eggNOG: prNOG08512 NOG268067
RefSeq: NP_003031.3   
Entrez Gene ID: 6522   
Pfam: PF07565    PF00955   
OMIM: 109280  gene
KEGG: hsa:6522    hsa:6522   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005624 C:membrane fraction
GO:0005452 F:inorganic anion exchanger activity
GO:0006820 P:anion transport
GO:0016323 C:basolateral plasma membrane
GO:0005886 C:plasma membrane
GO:0008509 F:anion transmembrane transporter activity
GO:0015108 F:chloride transmembrane transporter activity
GO:0015701 P:bicarbonate transport

References (16)

[1] “Complete nucleotide sequence of band 3 related anion transport protein AE2 from human kidney.”  Gehrig H.et.al.   1562608
[2] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[3] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[4] “Cloning and structural characterization of a human non-erythroid band 3-like protein.”  Demuth D.R.et.al.   3015590
[5] “A quantitative atlas of mitotic phosphorylation.”  Dephoure N.et.al.   18669648
[6] “Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.”  Gauci S.et.al.   19413330
[7] “Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.”  Mayya V.et.al.   19690332
[8] “Complete nucleotide sequence of band 3 related anion transport protein AE2 from human kidney.”  Gehrig H.et.al.   1562608
[9] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[10] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[11] “Cloning and structural characterization of a human non-erythroid band 3-like protein.”  Demuth D.R.et.al.   3015590
[12] “Multiple reaction monitoring for robust quantitative proteomic analysis of cellular signaling networks.”  Wolf-Yadlin A.et.al.   17389395
[13] “A quantitative atlas of mitotic phosphorylation.”  Dephoure N.et.al.   18669648
[14] “Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.”  Gauci S.et.al.   19413330
[15] “Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.”  Mayya V.et.al.   19690332
[16] “Initial characterization of the human central proteome.”  Burkard T.R.et.al.   21269460

External Searches:

  • Search: DB with
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  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSSAPRRPAK GADSFCTPEP ESLGPGTPGF PEQEEDELHR TLGVERFEEI LQEAGSRGGE 
61:	EPGRSYGEED FEYHRQSSHH IHHPLSTHLP PDARRRKTPQ GPGRKPRRRP GASPTGETPT 
121:	IEEGEEDEDE ASEAEGARAL TQPSPVSTPS SVQFFLQEDD SADRKAERTS PSSPAPLPHQ 
181:	EATPRASKGA QAGTQVEEAE AEAVAVASGT AGGDDGGASG RPLPKAQPGH RSYNLQERRR 
241:	IGSMTGAEQA LLPRVPTDEI EAQTLATADL DLMKSHRFED VPGVRRHLVR KNAKGSTQSG 
301:	REGREPGPTP RARPRAPHKP HEVFVELNEL LLDKNQEPQW RETARWIKFE EDVEEETERW 
361:	GKPHVASLSF RSLLELRRTL AHGAVLLDLD QQTLPGVAHQ VVEQMVISDQ IKAEDRANVL 
421:	RALLLKHSHP SDEKDFSFPR NISAGSLGSL LGHHHGQGAE SDPHVTEPLM GGVPETRLEV 
481:	ERERELPPPA PPAGITRSKS KHELKLLEKI PENAEATVVL VGCVEFLSRP TMAFVRLREA 
541:	VELDAVLEVP VPVRFLFLLL GPSSANMDYH EIGRSISTLM SDKQFHEAAY LADEREDLLT 
601:	AINAFLDCSV VLPPSEVQGE ELLRSVAHFQ RQMLKKREEQ GRLLPTGAGL EPKSAQDKAL 
661:	LQMVEAAGAA EDDPLRRTGR PFGGLIRDVR RRYPHYLSDF RDALDPQCLA AVIFIYFAAL 
721:	SPAITFGGLL GEKTQDLIGV SELIMSTALQ GVVFCLLGAQ PLLVIGFSGP LLVFEEAFFS 
781:	FCSSNHLEYL VGRVWIGFWL VFLALLMVAL EGSFLVRFVS RFTQEIFAFL ISLIFIYETF 
841:	YKLVKIFQEH PLHGCSASNS SEVDGGENMT WAGARPTLGP GNRSLAGQSG QGKPRGQPNT 
901:	ALLSLVLMAG TFFIAFFLRK FKNSRFFPGR IRRVIGDFGV PIAILIMVLV DYSIEDTYTQ 
961:	KLSVPSGFSV TAPEKRGWVI NPLGEKSPFP VWMMVASLLP AILVFILIFM ETQITTLIIS 
1021:	KKERMLQKGS GFHLDLLLIV AMGGICALFG LPWLAAATVR SVTHANALTV MSKAVAPGDK 
1081:	PKIQEVKEQR VTGLLVALLV GLSIVIGDLL RQIPLAVLFG IFLYMGVTSL NGIQFYERLH 
1141:	LLLMPPKHHP DVTYVKKVRT LRMHLFTALQ LLCLALLWAV MSTAASLAFP FILILTVPLR 
1201:	MVVLTRIFTD REMKCLDANE AEPVFDEREG VDEYNEMPMP V