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2.A.35.1.2
Malate + H+: lactate + Na+ antiporter, MleN

Accession Number:P54571
Protein Name:MleN aka YqkI
Length:468
Molecular Weight:50212.00
Species:Bacillus subtilis [1423]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate malate, H+, Lactate, Na+

Cross database links:

HEGENOM: HBG307651
RefSeq: NP_390237.1   
Entrez Gene ID: 938720   
Pfam: PF03553   
KEGG: bsu:BSU23560   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015385 F:sodium:hydrogen antiporter activity
GO:0006885 P:regulation of pH
GO:0006814 P:sodium ion transport

References (3)

[1] “Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes.”  Mizuno M.et.al.   8969508
[2] “The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.”  Kunst F.et.al.   9384377
[3] “Bacillus subtilis YqkI is a novel malic/Na+-lactate antiporter that enhances growth on malate at low protonmotive force.”  Wei Y.et.al.   10903309

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
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FASTA formatted sequence
1:	MKDVRLPTLF EIIIVLGVFL ALVLSFTVFL DLPIQLALFV SWFIAMLLGI RLGYSYKDLQ 
61:	NAIVHGISNG LEAVLILVSV GALIGTWIAG GVVPTLIYYG LEFIHPSIFL LATLIICSIM 
121:	SVATGTSWGT VGTAGIAMIA IGEGLGIPLP LVAGAILSGA YFGDKLSPLS DSTVLASSLS 
181:	KVDVLAHVRA MLYLSIPAYV ITAILFTVVG FMYGGKNIDL DKVEFLKSSL QNTFDIHIWM 
241:	LIPAVLVIVL LAMKKPSMPV IVIGALLGAI WAVVFQGMDI AHAIATAYNG FSIKTDVEFL 
301:	NGLLNRGGIV GMLDSLVVII FGLGFGGLLE KLGVLKVIVS TFEKKLTSAG NVTLSTLIVA 
361:	FLANIFGCAM YVSLILTPKI MEDSYDRLHL DRRVLSRNSE VGGTLTSGMV PWSDNGIYMA 
421:	GILGVSTFSY LPFMWLSFVA IGLAIIYGYT GKFIWYTKNN TVKAEKLG