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2.A.38.2.1
High affinity K+ transporter (K+:H+ symporter). Trk1 is functional without its "long hydrophilic loop", but this LHL regulates cation translocation activity and selectivity (Kale et al. 2019). Possibly, LHL influences a transmembrane helix (M2A) which can switch between bent and straight conformations, thereby directly modifying the radius of the pore and selectivity filter.

Accession Number:P12685
Protein Name:Trk1 aka YJL129C aka J0693
Length:1235
Molecular Weight:141073.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:9
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate K+, H+

Cross database links:

Genevestigator: P12685
eggNOG: fuNOG05343
HEGENOM: HBG398167
DIP: DIP-7454N
RefSeq: NP_012406.1   
Entrez Gene ID: 853312   
Pfam: PF02386   
KEGG: sce:YJL129C   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0045121 C:membrane raft
GO:0015079 F:potassium ion transmembrane transporter act...
GO:0030007 P:cellular potassium ion homeostasis
GO:0006813 P:potassium ion transport
GO:0055085 P:transmembrane transport

References (5)

[1] “TRK1 encodes a plasma membrane protein required for high-affinity potassium transport in Saccharomyces cerevisiae.”  Gaber R.F.et.al.   3043197
[2] “Sequencing analysis of a 40.2 kb fragment of yeast chromosome X reveals 19 open reading frames including URA2 (5' end), TRK1, PBS2, SPT10, GCD14, RPE1, PHO86, NCA3, ASF1, CCT7, GZF3, two tRNA genes, three remnant delta elements and a Ty4 transposon.”  Cziepluch C.et.al.   8948101
[3] “Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.”  Galibert F.et.al.   8641269
[4] “Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.”  Li X.et.al.   17330950
[5] “A multidimensional chromatography technology for in-depth phosphoproteome analysis.”  Albuquerque C.P.et.al.   18407956

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MHFRRTMSRV PTLASLEIRY KKSFGHKFRD FIALCGHYFA PVKKYIFPSF IAVHYFYTIS 
61:	LTLITSILLY PIKNTRYIDT LFLAAGAVTQ GGLNTVDINN LSLYQQIVLY IVCCISTPIA 
121:	VHSCLAFVRL YWFERYFDGI RDSSRRNFKM RRTKTILERE LTARTMTKNR TGTQRTSYPR 
181:	KQAKTDDFQE KLFSGEMVNR DEQDSVHSDQ NSHDISRDSS NNNTNHNGSS GSLDDFVKED 
241:	ETDDNGEYQE NNSYSTVGSS SNTVADESLN QKPKPSSLRF DEPHSKQRPA RVPSEKFAKR 
301:	RGSRDISPAD MYRSIMMLQG KHEATAEDEG PPLVIGSPAD GTRYKSNVNK LKKATGINGN 
361:	KIKIRDKGNE SNTDQNSVSS EANSTASVSD ESSLHTNFGN KVPSLRTNTH RSNSGPIAIT 
421:	DNAETDKKHG PSIQFDITKP PRKISKRVST FDDLNPKSSV LYRKKASKKY LMKHFPKARR 
481:	IRQQIKRRLS TGSIEKNSSN NVSDRKPITD MDDDDDDDDN DGDNNEEYFA DNESGDEDER 
541:	VQQSEPHSDS ELKSHQQQQE KHQLQQNLHR MYKTKSFDDN RSRAVPMERS RTIDMAEAKD 
601:	LNELARTPDF QKMVYQNWKA HHRKKPNFRK RGWNNKIFEH GPYASDSDRN YPDNSNTGNS 
661:	ILHYAESILH HDGSHKNGSE EASSDSNENI YSTNGGSDHN GLNNYPTYND DEEGYYGLHF 
721:	DTDYDLDPRH DLSKGSGKTY LSWQPTIGRN SNFLGLTRAQ KDELGGVEYR AIKLLCTILV 
781:	VYYVGWHIVA FVMLVPWIIL KKHYSEVVRD DGVSPTWWGF WTAMSAFNDL GLTLTPNSMM 
841:	SFNKAVYPLI VMIWFIIIGN TGFPILLRCI IWIMFKISPD LSQMRESLGF LLDHPRRCFT 
901:	LLFPKAATWW LLLTLAGLNI TDWILFIILD FGSTVVKSLS KGYRVLVGLF QSVSTRTAGF 
961:	SVVDLSQLHP SIQVSYMLMM YVSVLPLAIS IRRTNVYEEQ SLGLYGDMGG EPEDTDTEDD 
1021:	GNDEDDDEEN ESHEGQSSQR SSSNNNNNNN RKKKKKKKTE NPNEISTKSF IGAHLRKQLS 
1081:	FDLWFLFLGL FIICICEGDK IKDVQEPNFN IFAILFEIVS AYGTVGLSLG YPDTNQSFSR 
1141:	QFTTLSKLVI IAMLIRGKNR GLPYSLDRAI ILPSDRLEHI DHLEGMKLKR QARTNTEDPM 
1201:	TEHFKRSFTD VKHRWGALKR KTTHSRNPKR SSTTL