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2.A.4.7.1
Zn2+/Cd2+/Hg2+/Fe2+:H+ antiporter, YiiP or FieF (Chao and Fu, 2004b; Grass et al., 2005; Wei et al., 2004; Wei and Fu, 2006). The structure (3.8 Å resolution) reveals a homodimer interconnected at the cytoplasmic domain through four Zn2+ ions. A 6 TMS bundle features of a tetrahedral Zn2+ binding site (Lu and Fu, 2007). The gated water access to the transport site enables a stationary proton gradient to facilitate the conversion of zinc-binding energy to the kinetic power stroke of a vectorial zinc transport (Gupta et al. 2014). Of the two cavities of the dimer, only one was accessible from the cytosol during transport. Zinc(II) binding to D49 of the transport site triggered a rearrangement of the TMS that closed the accessible cavity. The free-energy profiles of metal transit in the channel suggested the existence of a high barrier preventing release from the transport site (Sala et al. 2019).

Accession Number:P69380
Protein Name:Ferrous-iron efflux pump fieF
Length:300
Molecular Weight:32927.00
Species:Escherichia coli [83333]
Number of TMSs:6
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Zn2+, Cd2+, Hg2+, Fe2+, H+

Cross database links:

Genevestigator: P69380
EchoBASE: EB1819
EcoGene: EG11873
eggNOG: COG0053
HEGENOM: HBG317426
RefSeq: AP_003894.1    NP_418350.1   
Entrez Gene ID: 948413   
Pfam: PF01545   
BioCyc: EcoCyc:YIIP-MONOMER    ECOL168927:B3915-MONOMER   
KEGG: ecj:JW3886    eco:b3915   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015086 F:cadmium ion transmembrane transporter activity
GO:0015093 F:ferrous iron transmembrane transporter acti...
GO:0015341 F:zinc efflux active transmembrane transporte...
GO:0015691 P:cadmium ion transport
GO:0006876 P:cellular cadmium ion homeostasis
GO:0006879 P:cellular iron ion homeostasis
GO:0006882 P:cellular zinc ion homeostasis
GO:0015684 P:ferrous iron transport
GO:0055085 P:transmembrane transport
GO:0006829 P:zinc ion transport

References (6)

[1] “Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes.”  Plunkett G. IIIet.al.   8346018
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “Oligomeric state of the Escherichia coli metal transporter YiiP.”  Wei Y.et.al.   15258151
[5] “FieF (YiiP) from Escherichia coli mediates decreased cellular accumulation of iron and relieves iron stress.”  Grass G.et.al.   15549269
[6] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996
Structure:
2QFI   3H90     

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FASTA formatted sequence
1:	MNQSYGRLVS RAAIAATAMA SLLLLIKIFA WWYTGSVSIL AALVDSLVDI GASLTNLLVV 
61:	RYSLQPADDN HSFGHGKAES LAALAQSMFI SGSALFLFLT GIQHLISPTP MTDPGVGVIV 
121:	TIVALICTII LVSFQRWVVR RTQSQAVRAD MLHYQSDVMM NGAILLALGL SWYGWHRADA 
181:	LFALGIGIYI LYSALRMGYE AVQSLLDRAL PDEERQEIID IVTSWPGVSG AHDLRTRQSG 
241:	PTRFIQIHLE MEDSLPLVQA HMVADQVEQA ILRRFPGSDV IIHQDPCSVV PREGKRSMLS