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2.A.45.2.4
The plasma membrane silicon (silicate) efflux pump, Low Silicon 2, Lsi2 (localized to the proximal side of the cell, whereas the silicon uptake system, Lsi1 (TC# 1.A.8.12.2) is localized to the distal side of the same cells) (Ma et al., 2007a, b).

Accession Number:Q10SY9
Protein Name:Transmembrane protein, putative, expressed aka Lsi2
Length:472
Molecular Weight:50359.00
Species:Oryza sativa subsp. japonica (Rice) [39947]
Number of TMSs:10
Location1 / Topology2 / Orientation3: Plastid1 / Multi-pass membrane protein2
Substrate Silicate

Cross database links:

HEGENOM: HBG727257
RefSeq: NP_001048691.1   
Entrez Gene ID: 4331328   
Pfam: PF03600   
KEGG: osa:4331328   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0015137 F:citrate transmembrane transporter activity
GO:0015746 P:citrate transport
GO:0055085 P:transmembrane transport

References (4)

[1] “Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species.”  Buell C.R.et.al.   16109971
[2] “An efflux transporter of silicon in rice.”  Ma J.F.et.al.   17625566
[3] “The genomes of Oryza sativa: a history of duplications.”  Yu J.et.al.   15685292
[4] “The Rice Annotation Project Database (RAP-DB): hub for Oryza sativa ssp. japonica genome information.”  Ohyanagi H.et.al.   16381971

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSELASAPKV ALGSIAFAVF WMMAVFPSVP FLPIGRTAGS LLSAVLMVIF HVISPDDAYA 
61:	SIDLPILGLL FATMVVGSYL RNAGMFKHLG RLLAWKSQGG RDLMCRVCVV TALASALFTN 
121:	DTCCVVLTEF VLELAAERNL PAKPFLLALA SSANIGSAAT PIGNPQNLVI AFNSKITFPK 
181:	FLMGILPAML VGMAVNMVML LCMYWRELGG GAELSVDGKQ MEAVEEGRSP ASAKSTPQLN 
241:	GNGNTMMSLE MSENITTKHP WFMQCTEARR KLFLKSFAYV VTVGMVVAYM VGLNMSWTAI 
301:	TTALALVVVD FRDAEPCLDT VSYSLLVFFS GMFITVSGFN KTGLPGAIWD FMAPYSKVNS 
361:	VGGISVLSVI ILLLSNLASN VPTVLLMGDE VAKAAALISP AAVTTSWLLL AWVSTVAGNL 
421:	SLLGSAANLI VCEQARRAPR NAYDLTFWQH IVFGVPSTLI VTAVGIPLIG KI