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2.A.48.1.1
Folate/folate derivative transporter, RFC1. Although oligomeric, each monomer provides the channel (Hou et al., 2010). Downregulated in Chronic Kidney Disease (CKD) in heart, liver, and brain causing malabsorption (Bukhari et al., 2011).  5-Aminoimidazole-4-carboxamide riboside (AICAR), an agent with diverse pharmacological properties, augments transport of folates and antifolates by promoting substrate:substrate antiport (Visentin et al. 2012).  Its role in antifolate cancer chemotherapy has been reviewed (Matherly et al. 2014).

Accession Number:P41440
Protein Name:Folate transporter 1
Length:591
Molecular Weight:64868.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate folate, Folate derivatives

Cross database links:

NOG241030
Genevestigator: P41440 P41440
HEGENOM: HBG385651
RefSeq: NP_919231.1   
Entrez Gene ID: 6573   
Pfam: PF01770   
OMIM: 600424  gene
KEGG: hsa:6573    hsa:6573   

Gene Ontology

GO:0005887 C:integral to plasma membrane
GO:0005624 C:membrane fraction
GO:0005542 F:folic acid binding
GO:0008517 F:folic acid transporter activity
GO:0015350 F:methotrexate transporter activity
GO:0008518 F:reduced folate carrier activity
GO:0015884 P:folic acid transport
GO:0046655 P:folic acid metabolic process

References (20)

[1] “Molecular cloning of the human placental folate transporter.”  Prasad P.D.et.al.   7826387
[2] “Isolation of human cDNAs that restore methotrexate sensitivity and reduced folate carrier activity in methotrexate transport-defective Chinese hamster ovary cells.”  Wong S.C.et.al.   7615551
[3] “Isolation of a gene encoding a human reduced folate carrier (RFC1) and analysis of its expression in transport-deficient, methotrexate-resistant human breast cancer cells.”  Moscow J.A.et.al.   7641195
[4] “Isolation of a human cDNA that complements a mutant hamster cell defective in methotrexate uptake.”  Williams F.M.et.al.   7852378
[5] “Human intestinal folate transport: cloning, expression, and distribution of complementary RNA.”  Nguyen T.T.et.al.   9041240
[6] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[7] “The DNA sequence of human chromosome 21.”  Hattori M.et.al.   10830953
[8] “Topological and functional analysis of the human reduced folate carrier by hemagglutinin epitope insertion.”  Ferguson P.L.et.al.   10347183
[9] “A quantitative atlas of mitotic phosphorylation.”  Dephoure N.et.al.   18669648
[10] “Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.”  Gauci S.et.al.   19413330
[11] “Molecular cloning of the human placental folate transporter.”  Prasad P.D.et.al.   7826387
[12] “Isolation of human cDNAs that restore methotrexate sensitivity and reduced folate carrier activity in methotrexate transport-defective Chinese hamster ovary cells.”  Wong S.C.et.al.   7615551
[13] “Isolation of a gene encoding a human reduced folate carrier (RFC1) and analysis of its expression in transport-deficient, methotrexate-resistant human breast cancer cells.”  Moscow J.A.et.al.   7641195
[14] “Isolation of a human cDNA that complements a mutant hamster cell defective in methotrexate uptake.”  Williams F.M.et.al.   7852378
[15] “Human intestinal folate transport: cloning, expression, and distribution of complementary RNA.”  Nguyen T.T.et.al.   9041240
[16] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[17] “The DNA sequence of human chromosome 21.”  Hattori M.et.al.   10830953
[18] “Topological and functional analysis of the human reduced folate carrier by hemagglutinin epitope insertion.”  Ferguson P.L.et.al.   10347183
[19] “A quantitative atlas of mitotic phosphorylation.”  Dephoure N.et.al.   18669648
[20] “Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.”  Gauci S.et.al.   19413330

External Searches:

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FASTA formatted sequence
1:	MVPSSPAVEK QVPVEPGPDP ELRSWRHLVC YLCFYGFMAQ IRPGESFITP YLLGPDKNFT 
61:	REQVTNEITP VLSYSYLAVL VPVFLLTDYL RYTPVLLLQG LSFVSVWLLL LLGHSVAHMQ 
121:	LMELFYSVTM AARIAYSSYI FSLVRPARYQ RVAGYSRAAV LLGVFTSSVL GQLLVTVGRV 
181:	SFSTLNYISL AFLTFSVVLA LFLKRPKRSL FFNRDDRGRC ETSASELERM NPGPGGKLGH 
241:	ALRVACGDSV LARMLRELGD SLRRPQLRLW SLWWVFNSAG YYLVVYYVHI LWNEVDPTTN 
301:	SARVYNGAAD AASTLLGAIT SFAAGFVKIR WARWSKLLIA GVTATQAGLV FLLAHTRHPS 
361:	SIWLCYAAFV LFRGSYQFLV PIATFQIASS LSKELCALVF GVNTFFATIV KTIITFIVSD 
421:	VRGLGLPVRK QFQLYSVYFL ILSIIYFLGA MLDGLRHCQR GHHPRQPPAQ GLRSAAEEKA 
481:	AQALSVQDKG LGGLQPAQSP PLSPEDSLGA VGPASLEQRQ SDPYLAQAPA PQAAEFLSPV 
541:	TTPSPCTLCS AQASGPEAAD ETCPQLAVHP PGVSKLGLQC LPSDGVQNVN Q