TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


2.A.66.1.23
MDR efflux pump, YeeO (NorA) (81.8% identical to 2.A.66.1.22). Transports dipeptides (see 2.A.1.2.55) (Hayashi et al., 2010).  Also exports both flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). However, significant amounts of flavins were trapped intracellularly when YeeO was produced. Wild-type E. coli secretes 3 flavins (riboflavin, FMN, and FAD), so it must have additional flavin transporters (McAnulty and Wood 2014).

Accession Number:P76352
Protein Name:Uncharacterized transporter yeeO
Length:495
Molecular Weight:54199.00
Species:Escherichia coli (strain K12) [83333]
Number of TMSs:12
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate dipeptides, FMN, FAD

Cross database links:

Genevestigator: P76352 P76352
EchoBASE: EB3164 EB3164
EcoGene: EG13383 EG13383
eggNOG: COG0534 COG0534
HEGENOM: HBG698717 HBG698717
Entrez Gene ID: 946506   
Pfam: PF01554   
KEGG: ecj:JW1965    eco:b1985    ecj:JW1965    eco:b1985   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015297 F:antiporter activity
GO:0015238 F:drug transmembrane transporter activity

References (6)

[1] “A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.”  Itoh T.et.al.   9097040
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map.”  Itoh T.et.al.   9097040
[5] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[6] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MWFHFLQLRS ALNISSALRQ VVHGTRWHAK RKSYKVLFWR EITPLAVPIF MENACVLLMG 
61:	VLSTFLVSWL GKDAMAGVGL ADSFNMVIMA FFAAIDLGTT VVVAFSLGKR DRRRARVATR 
121:	QSLVIMTLFA VLLATLIHHF GEQIIDFVAG DATTEVKALA LTYLELTVLS YPAAAITLIG 
181:	SGALRGAGNT KIPLLINGSL NILNIIISGI LIYGLFSWPG LGFVGAGLGL TISRYIGAVA 
241:	ILWVLAIGFN PALRISLKSY FKPLNFSIIW EVMGIGIPAS VESVLFTSGR LLTQMFVAGM 
301:	GTSVIAGNFI AFSIAALINL PGSALGSAST IITGRRLGVG QIAQAEIQLR HVFWLSTLGL 
361:	TAIAWLTAPF AGVMASFYTQ DPQVKHVVVI LIWLNALFMP IWSASWVLPA GFKGARDARY 
421:	AMWVSMLSMW GCRVVVGYVL GIMLGWGVVG VWMGMFADWA VRAVLFYWRM VTGRWLWKYP 
481:	RPEPQKCEKK PVVSE